Past seminars since 2016

Location: MPI for Terrestrial Microbiology

Revving up: The dynamics of bacterial flagellar stators

Microbiology Seminar Series
Cytochrome P450 enzymes are catalysts of unrivalled versatility, able to mediate over 60 different monooxygenation and other reactions, such as aromatic and aliphatic hydroxylation at unactivated C-H bonds, epoxidation of olefins and aromatic rings, heteroatom oxidation and dealkylation, C-C bond cleavage, ring rearrangements, isomerizations and reductions. This catalytic versatility has led to the exploitation of P450s across the biosphere, for diverse functions such as the mobilization of carbon sources, chemical communication (e.g. hormonal signalling), and inter-organismal chemical warfare, such as between plants and the herbivores that feed on them. They were pivotal for the transition from water to land by plants and animals and play central roles in natural product biosynthesis in microbes and secondary metabolism in plants. This versatility also makes them attractive for catalyzing industrially important reactions in pharmaceutical and other fine chemical syntheses. However, P450 enzymes from natural sources are limited by poor stability and the need for accessory enzymes and a reducing cofactor. Moreover, expression of P450s in recombinant systems typically requires rich media, all of which increases the cost of using P450s for industrial biocatalysis. We have used ancestral sequence reconstruction as a technique for both engineering P450s as biocatalysts and exploring their evolution. This presentation will look both backwards and forwards. Using examples drawn from reconstructions of plant, animal and microbial P450 families, I will discuss what we have learned about the natural evolution of these enzymes. Then, I will show how ancestral P450s can be used as cost-effective, modular bio-bricks for synthetic biology, to create biocatalytic systems powered by photosynthesis and nanobioreactors based on P450s in virus-like particles. Cytochrome P450 enzymes are catalysts of unrivalled versatility, able to mediate over 60 different monooxygenation and other reactions, such as aromatic and aliphatic hydroxylation at unactivated C-H bonds, epoxidation of olefins and aromatic rings, heteroatom oxidation and dealkylation, C-C bond cleavage, ring rearrangements, isomerizations and reductions. This catalytic versatility has led to the exploitation of P450s across the biosphere, for diverse functions such as the mobilization of carbon sources, chemical communication (e.g. hormonal signalling), and inter-organismal chemical warfare, such as between plants and the herbivores that feed on them. They were pivotal for the transition from water to land by plants and animals and play central roles in natural product biosynthesis in microbes and secondary metabolism in plants. This versatility also makes them attractive for catalyzing industrially important reactions in pharmaceutical and other fine chemical syntheses. However, P450 enzymes from natural sources are limited by poor stability and the need for accessory enzymes and a reducing cofactor. Moreover, expression of P450s in recombinant systems typically requires rich media, all of which increases the cost of using P450s for industrial biocatalysis. We have used ancestral sequence reconstruction as a technique for both engineering P450s as biocatalysts and exploring their evolution. This presentation will look both backwards and forwards. Using examples drawn from reconstructions of plant, animal and microbial P450 families, I will discuss what we have learned about the natural evolution of these enzymes. Then, I will show how ancestral P450s can be used as cost-effective, modular bio-bricks for synthetic biology, to create biocatalytic systems powered by photosynthesis and nanobioreactors based on P450s in virus-like particles. [more]

Graduate Students Mini Symposium VII - 2024

Graduate Students Mini-Symposium

Elucidate the mechanism of PhoQ inhibition by the small protein MgrB

Doctoral thesis defense

The effect of tRNA structure and codon context on translation efficiency and fidelity

Microbiology Seminar Series

Ask the ancestors: resurrecting and re-evolving the bacterial flagellar motor

Guest Speaker Talk

Steinernema nematodes as an emerging genetic model to study microbial symbiosis

Microbiology Seminar Series

Development and characterization of new tools for advanced SMLM imaging schemes

Doctoral thesis defense

Biosyntheses of natural products from Proteobacteria

Doctoral thesis defense

Bacteriophages reinvented: tiny killers, life savers and detectives

Microbiology Seminar Series

The type VI secretion systems of Pseudomonas aeruginosa

Microbiology Seminar Series

Scientific writing: Turning the blank page into a manuscript

Workshop

Uncovering transcriptional heterogeneity during Vibrio cholerae biofilm development

Doctoral thesis defense

Graduate Students Mini Symposium III-2024

Graduate Students Mini-Symposium

Genetic circuits on single DNA molecules, with and without cell-like compartments

Microbiology Seminar Series

Graduate Students Mini Symposium II-2024

Graduate Students Mini-Symposium

Synthetic membranes, synthetic cells: Exploring the new frontier of cell engineering with polymers

Microbiology Seminar Series

Graduate Students Mini Symposium I-2024

Graduate Students Mini-Symposium

Establishment of a microfluidic setup to characterize whole cell biosensors for bacteriocin detection

Guest Speaker Talk

Evolution of essential complexity in Rubisco

Doctoral thesis defense

Graduate Students Mini Symposium XI-2023

Graduate Students Mini-Symposium

Genetic and non-genetic solutions to survive a lethal metabolic stress in Methylobacterium extorquens

Microbiology Seminar Series

Insights into assembly of the type IVa pilus machine in Myxococcus xanthus

Doctoral thesis defense

Why is E. coli's brain tuned so close to criticality?

Microbiology Seminar Series

Graduate Students Mini Symposium X-2023

Graduate Students Mini-Symposium

Evolution of molecular innovations in cyanobacterial light-perceiving systems

Doctoral thesis defense

Graduate Students Mini Symposium IX-2023

Graduate Students Mini-Symposium

Engineering yeast for methanol-based biomanufacturing

Methanol is an ideal feedstock for bio-manufacturing that can be beneficial for global carbon neutrality. However, the toxicity of methanol limits the efficiency of methanol metabolism toward biochemical production, and it is still challenging in engineering this non-conventional yeast due to serious lack of genetic editing tools. In this presentation, we will show our recent progress in establishing CRISPR-Cas9 based genome editing tools and enhancing the homologous recombination in methylotrophic yeast Ogataea polymorpha. With this genetic platform, we tried to engineer cellular metabolism for fatty acid production from methanol. We found that engineering overproduction of free fatty acids (FFA) from sole methanol resulted cell death with a decreased cellular phospholipid in O. polymorpha, and the cell growth was restored by adaptive laboratory evolution (ALE). Whole genome sequencing of the adapted strains reveals that inactivation of LPL1 (encoding a putative lipase) and IZH3 (encoding a membrane protein related to zinc metabolism) preserve cell survival by restoring phospholipid metabolism. Engineering the pentose phosphate pathway and gluconeogenesis enabled high-level production of FFA (15.9 g/L) from sole methanol. Preventing methanol-associated toxicity underscored the link between lipid metabolism and methanol tolerance, which should contribute to enhancing methanol-based bio-manufacturing. [more]

The evolution of the molecular toolkit for pluripotent stem cells

We use native and re-designed versions of SOX and OCT transcription factors to reprogram somatic cells into induced pluripotent stem cells and for stem cell engineering. In mammals, what distinguishes paralogous SOX factors is their ability to dimerize with OCT4 on distinctive composite DNA elements to induce stemness (SOX2) or specify the germline (SOX17). The selective partnership of these two SOX factors with OCT4 is primarily directed by a single amino acid conserved across animals. Mutating this amino acid converts SOX17 into an enhanced 'super SOX2', termed eSOX17, which speeds up pluripotency induction. In human cells, eSOX17 enables the direct conversion of somatic cells into totipotent cells. In two-factor cocktails, eSOX17 can transdifferentiate human blood into induced brain stem cells, avoiding the rejuvenation associated with pluripotency induction. Inspired by the close partnership between SOX/OCT and the potential of re-engineered variants for cellular reprogramming, we have begun to study their evolutionary history. Until now, SOX and OCT factors have been believed to be unique to animals. Surprisingly, we identified these factors in certain lineages of unicellular holozoans and found that they can replace SOX2 to induce pluripotency in mice. It is possible that the emergence of the molecular toolkit essential for mammalian pluripotency predates the evolution of multicellularity. [more]

A mechanistic understanding of bacterial chromosome organisation

Doctoral thesis defense

Graduate Students Mini Symposium VIII - 2023

Graduate Students Mini-Symposium

Evolution of evolvability: adaptive hyper mutability by lineage selection

Microbiology Seminar Series

The intricate machinery of the bacterial flagellar motor and its regulation

Guest Speaker Talk
The bacterial flagellar motor is a membrane-embedded rotary macromolecular machine that converts the electrochemical energy of the proton gradient into the mechanical energy of rotation. The knowledge about the bacterial motor is a source of inspiration for nanotechnology and one of the first steps towards making artificial motors on the same scale. Recent breakthrough electron cryotomography studies have revealed proteinaceous periplasmic structures adjacent to the stator (the powerhouse) of polar flagellar motors, which are essential for the stator assembly and function. The talk will showcase the cutting-edge research on the structure, composition, and function of the periplasmic scaffold in the polar bacterial flagellar motor of Helicobacter pylori. This microorganism displays high motility in the very viscous mucous layer of the stomach, which enables us to use H. pylori as a model system to study the polar motor specialised for locomotion in highly viscous fluids. The presented work will illustrate the advantages of an interdisciplinary approach combining biology and physics. The presentation will conclude with the discussion of the new paradigm for how the previously unseen accessory components control the function of the flagellar motor. [more]

Game of Tug-of-war: ParA2 oscillations spatially regulates Vibrio cholerae chromosome 2 segregation

Microbiology Seminar Series

Graduate Students Mini Symposium VII - 2023

Graduate Students Mini-Symposium

Nitrogen fixation in cyanobacteria: Mechanism for coexist of oxygen-sensitive enzymes and oxygenic photosynthesis

Guest Speaker Talk

Towards the extension of the substrate spectrum of the [Fe]-hydrogenase Hmd

Doctoral thesis defense

Hydride intermediates in the catalytic turnover of gas-processing metalloenzymes from diverse microorganisms

Microbiology Seminar Series

Graduate Students Mini Symposium VI - 2023

Graduate Students Mini-Symposium

A metabolic portrait of the developing bacterial endospore

Microbiology Seminar Series

More is Different: Membrane Protein Function as a Collective Phenomenon

Microbiology Seminar Series

Engineering enzymes and pathways for alternative CO2 fixation and glyoxylate assimilation

Doctoral thesis defense

Graduate Students Mini Symposium V - 2023

Graduate Students Mini-Symposium

Non-canonical molecular biology of bacteriophages

Microbiology Seminar Series

Roots and bacteria: Basis of attraction

Microbiology Seminar Series

Evolution of evolvability: adaptive hyper mutability by lineage selection

Microbiology Seminar Series

Design and directed evolution of artificial lanthanide enzymes

Microbiology Seminar Series

Graduate Students Mini Symposium IV-2023

Graduate Students Mini-Symposium

Determinants and evolution of metabolic interactions in synthetic microbial communities

Doctoral thesis defense

Investigating the kinetochore complex in Schizosaccharomyces pombe using advanced fluorescence microscopy techniques

Doctoral thesis defense

Protein transport in bacteria: multiple pathways and common concepts

Microbiology Seminar Series

Enzymatic systems for synthetic formate assimilation

Doctoral thesis defense

Graduate Students Mini Symposium III-2023

Graduate Students Mini-Symposium

The impact of differential temperatures (30°C versus 45°C) on the methanogenic community in Philippine rice field soil

Doctoral thesis defense

Graduate Students Mini Symposium II 2023

Microbiology Seminar Series

Graduate Students Mini Symposium I 2023

Microbiology Seminar Series

Protein diffusion in the cytoplasm of Escherichia coli

Doctoral thesis defense

How to catch a nematode if you were a mushroom

Microbiology Seminar Series

The predation strategy of Myxococcus xanthus

Microbiology Seminar Series

Graduate Students Mini Symposium VI 2020

Coming in for a landing: bacterial signaling pathways activated upon surface contact

SFB/Transregio TRR 174

Implant Infection and Innate Immunity

Microbiology Seminar Series

Comprehensive phylogenetic study of ECF sigma factors

PhD Defense

Why was a complement inhibitor used in the 2011 German EHEC outbreak?

SFB/Transregio TRR 174

The coming of age of de novo protein design

Microbiology Seminar Series

Engineering bioremediation agents: from the test tube to planet Earth

Microbiology Seminar Series

Graduate Students Mini Symposium V 2020 (virtual meeting)

IMPRS Selection Symposium

From stress to success: how actinobacteria exploit life without a cell wall

SFB/Transregio TRR 174

Graduate Students Mini Symposium IV 2020 (virtual meeting)

From insects to plants - Phenotypic switching of insect pathogenic Photorhabdus luminescens bacteria

SFB 987 Mini-Symposium

New bacterial degradation pathways for natural, environmental and intestinal organosulfonates

SFB 987 Mini-Symposium

Graduate Students Mini Symposium II & III 2020

Learning from predatory bacteria: from `omics´ to molecular mechanisms

Microbiology Seminar Series

Coordination of cell wall homeostasis with cell division: Genes, suppressors and beyond

SFB/Transregio TRR 174

Gene transfer agent: Evolution, function, and role in bacterial adaptation

SFB 987 Mini-Symposium

Photosynthetic Life in Hot Acid - Genomic and Physiological Insights into Extremophily in the Cyanidiales

SFB 987 Mini-Symposium

Production and analysis of synthetic cascade variants

PhD Defense

Apoplastic effectors in maize - U.maydis interface

Regulation of development by c-di-GMP in Myxococcus xanthus

PhD Defense

Graduate Students Mini Symposium I 2020

Microfluidic single-cell cultivation: Concept, application and challenges

Microbiology Seminar Series

Development of molecular tools for research on red and green algae

SFB 987 Mini-Symposium

Breaching the Barrier: Quantifying Antibiotic Permeability across Gram-negative Bacterial Membranes

SFB 987 Mini-Symposium

Kicking the"BolA" in Bacterial Survival and Virulence

SFB 987 Mini-Symposium

Mechanisms of microbiota-pathogen interactions

SFB 987 Mini-Symposium

Graduate Students Mini Symposia III and IV / 2019

Salmonella-microbiome interactions in the gut and their impact on transmission

Microbiology Seminar Series

Pseudomonas aeruginosa transcription profiling

SFB 987 Mini-Symposium

Exploration of Microbes in Animals, the Environment, and Beer

Special seminar

The bacterial stringent response in the context of root nodule symbiosis

SFB 987 Mini-Symposium

Disulfide bond formation underpins antimicrobial resistance in Enterobacteria

Microbiology Seminar Series

Bacterial "gap junctions" in cell-cell communication of multicellular cyanobacteria

SFB/Transregio TRR 174

Faculty of 1000 at Max Planck Institutes

Special seminar

Visualizing Bacterial Physiology at High Resolution using Single-Molecule Tracking and Lattice-Light Sheet Microscopy

A passion for biochemistry and microorganisms

Special Event

Transcriptional regulators in the archaeon Sulfolobus acidocaldarius: homologies and differences with bacterial regulators

Microbiology Seminar Series

Characterization of DNA interference by a minimal Type I-F CRISPR-Cas system

PhD Defense

Attempts towards the microbiological production of the second generation biofuel octanol

Special seminar

Probing the cofactor specificity, pH effect, and temperature effect of archaeal ketol-acid reductoisomerase by cryo-EM

Special seminar

Powering LPS transport across the bacterial cell envelope with ABCs

Transregio TRR 174 Seminar

STUDIES OF SINGLE-MOLECULE DYNAMICS IN MICROORGANISMS

PhD Defense

Graduate Students Mini-Symposium II/2019

Graduate Students Mini-Symposium

Molecular mechanism of sulfur mobilization toward Fe-S cluster biosynthesis

Special seminar

Managing the bacterial chromosome

Transregio TRR 174 Seminar

Dynamics of bacterial biofilm predation

PhD Defense

From biofuels to biotherapeutics - Engineering metalloenzymes for a better future

Microbiology Seminar Series

Protecting the offspring – Linking developmental pathways of Aspergillus fumigatus to toxic compounds

SFB 987 Mini-Symposium

How to get published in Nature Communications and other Nature journals

Special seminar

Specific integration and regulation of the prokaryotic-type protein synthesis machinery in chloroplasts of plants

Microbiology Seminar Series

Synthetic genomics: from genetic parts to genomes

Transregio TRR 174 Seminar

Surfing in the storm: Revealing biochemical networks of Burkholderiales to deal with toxic compounds and heavy metals

Special seminar

The Clockwork of Epigenetic Aging in Mammals

Special seminar

Sulfur incorporation into tRNA: Unique mechanistic features and functions in bacteria

SFB 987 Mini-Symposium

Replication control of multiple chromosomes in bacteria

Transregio TRR 174 Seminar

Computational modeling of metabolism in a minimal cell

Special seminar

Mechanosensing in cell membranes

Special seminar

Fungal-bacterial mutualistic interaction; fungal highway and bacterial toll

Mass spectrometric exploration of the proteotype

Microbiology Seminar Series

The evolution and functions of gut microbiota in bees

SFB 987 Mini-Symposium

Defining species in the microbial world

SFB 987 Mini-Symposium

Synthetic Gene-Metabolic Circuits for Bioproduction, Biosensing and Biocomputation

Special seminar

Robust and promiscuous monooxygenase biocatalysts - From discovery to design

Special seminar

Microbial mediation of folivory

Special seminar

Self-organization of the bacterial cell division machinery

Microbiology Seminar Series

Salmonella diarrhea: mucus barriers, hydrogen-fueled growth and DNA transfer in the gut

Microbiology Seminar Series

Permutation of Split Protein Cages

Special seminar

Cryo-EM gets sweet: Molecular insight into glycosylation

Special seminar

Toward artificial cells: Engineering synthetic membranes, organelles, gene circuits and communication

Special seminar

The burden of inherited interfaces: evolution of self-assembly after gene duplication

Microbiology Seminar Series

The ParA/MinD family of ATPases make waves to position DNA, Cell Division, & Organelles in bacteria

Special seminar

The MocR-like transcription factors: pyridoxal 5’-phosphate-dependent regulators of bacterial metabolism

SFB 987 Mini-Symposium

"It-Ma(t)Ter(s)" Conference

This is a joint young researcher's conference organized between the MPI for Terrestrial Microbiology and our partner MPI for Marine Microbiology in Bremen. [more]

Targeted Intracellular Delivery of Enzymes with Engineered AB5 Carriers

Special seminar

Unipolar growth of Brucella abortus in culture and inside host cells

Transregio TRR 174 Seminar

An optimized formate-assimilation pathway via enzyme engineering.

Special seminar

Understanding and exploiting bacterial lifestyles

Transregio TRR 174 Seminar

Pathogen effectors hitchhike the host ubiquitination pathway

SFB 987 Mini-Symposium

Towards synthetic life: Establishing a minimal segrosome for the rational design of biomimetic systems

PhD Defense

### Cancelled ###

Microbiology Seminar Series

To be announced

Transregio TRR 174 Seminar

Minimal metabolic engineering - getting the most bang for your buck

SFB 987 Mini-Symposium

Graduate Students Mini Symposium I/2019

Graduate Students Mini-Symposium

Organelle-like structures in Agrobacterium tumefaciens and Ralstonia eutropha

Transregio TRR 174 Seminar

A protein complex formed by Ustilago maydis effectors is essential for virulence (PhD Defense)

PhD Defense

A new facet of vitamin B12: gene regulation by a novel and widespread family of adenosylcobalamin-dependent photoreceptors in bacteria

SFB 987 Mini-Symposium

Salmonella persisters during infection

C A N C E L E D - SFB 987 Mini-Symposium

The ParC/ ParP system in the localization and segregation of chemotaxis signaling arrays in Vibrio cholerae and Vibrio parahaemolyticus (PhD Defense)

PhD Defense

Graduate Students Mini-Symposium VI/2018

Graduate Students Mini-Symposium

Understanding cell division and its regulation in the human pathogenic bacterium, Vibrio parahaemolyticus (PhD Defense)

PhD Defense

Frontiers in Microbiology 2018

Special seminar

Frontiers in Microbiology 2018

Special seminar

RocS drives chromosome segregation and nucleoid protection during cell division of Streptococcus pneumoniae

Transregio TRR 174 Seminar

ChAP1, a transducer of oxidant and small-molecule signals in the maize pathogen Cochliobolus heterostrophus

SFB 987 Mini-Symposium

Mechanisms of co-translational folding and assembly of proteins studied by ribosome profiling

SFB 987 Mini-Symposium

Symposium: “Microbial Metabolomics”

Special Event

Darwin’s invertebrates: A transient anoxic microbial oasis

MPI Seminar

Antigen 43-mediated biotin display and fabrication of bacteria-driven microswimmers (PhD Defense)

PhD Defense

Structural and mechanistic insights into the guanine nucleotide exchange factor complex Mon1-Ccz1

SFB 987 Mini-Symposium

Predator-prey interactions between the nematode-trapping fungi and nematodes

The networking of microbiomes across plant generations

SFB 987 Mini-Symposium

Methylotrophic methanogens everywhere - ecology and physiology of novel players in global methane cycling

SFB 987 Mini-Symposium

Structural insights into effector kinases from pathogenic gram-negative bacteria

SFB 987 Mini-Symposium

A Time to Kill: Mechanisms and Biological Insights of T6SS-Mediated Bacterial Warfare

Identification and characterization of RomX and RomY, two novel motility regulators in Myxococcus xanthus

PhD Defense

Max-Planck-Tag 2018

Special Event

The role of M23 peptidases on cell division and cell shape in Vibrio parahaemolyticus

Graduate Students Mini-Symposium

Regulatory circuits controlling the glycine betaine synthesizing pathway in Bacillus subtilis

Graduate Students Mini-Symposium

Proteomics - a new tool for type II methanotroph research

Graduate Students Mini-Symposium
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