Graduate Students Mini Symposium IV 2020 (virtual meeting)

Program:

13:00 Richard McLean, AG Erb

Design and Realisation of the Synthetic Hydroxypropionyl-CoA/Acrylyl-CoA Cycle for Carbon Fixation

Abstract: The goal of this project is to bring a synthetic carbon-fixing cycle, which was designed by metabolic retrosynthesis, to life. The Hydroxypropionyl-CoA/Acrylyl-CoA (HOPAC) Cycle proceeds through a series of acyl-CoA intermediates and consumes two NADPH and three ATP while fixing two carbon, producing the C2 compound glyoxylate, and regenerating its substrate. The project takes an iterative approach to producing a functional cycle. The cycle is run, issues are identified, those issues are addressed, and the cycle is run again to determine what, if any, new issues arise.

13:30 Xiaohan Guo, AG Randau

Functional characterization of type IV CRISPR-Cas systems

Type IV CRISPR-Cas systems are known to be the “mysterious” CRISPR systems, these systems are often found missing adaptation modules and nucleases conduct target cleavage. In this project we identified a type IV CRISPR-Cas system shows PAM-dependent interference activity in vivo. The interference inhibit antibiotic resistance carried in the targeted plasmids. This interference depends on the putative helicase activity of DinG.