Microscopy
Epi-fluorescence microscopy
Leica DMI8 fluorescence widefield microscope with Hamamatsu Orca Flash 4.0 sCMOS camera.
For live-cell imaging with temperature control, black incubation chamber. With fluorescence lamp and lasers (488nm, 561nm) for fluorescence picture aquisition. Total internal reflection fluorescence microscopy (TIRF) mode and W-VIEW Gemini for real-time Förter Resosnanz Energy Transfer (FRET) experiments. Leica LAS X software for picture aquisition and analysis. With 10x, 40x, 63x and two 100x objectives for phase contrast and DIC microscopy.
Leica DMI8 fluorescence widefield microscope with Hamamatsu Orca Flash 4.0 sCMOS camera.
For live-cell imaging with temperature control, black incubation chamber. Leica LAS X software for picture aquisition and analysis. With 4x, 10x, 20x, 40x, 63x and a 100x objective for phase contrast microscopy.
Leica DMI600B fluorescence widefield microscope with Hamamatsu Orca Flash 4.0 sCMOS camera.
For live-cell imaging with temperature control, black incubation chamber and ProScan III motorized stage (Prior). Leica Metamorph AF software for picture aquisition and analysis. With 10x, 40x, 63x and two 100x obejctives for phase contrast and DIC microscopy
Leica M205FA binocular with fluorescence light source and Hamamatsu Orca Flash 4.0 sCMOS camera.
GE Deltavision Elite deconvolution/TIRF microscope
Ultimate focus system, laser module for TIRF and photobleaching applications, temperature controlled incubation chamber.
Confocal microscopy
Nikon eclipse Ti inverted epifluorescence and confocal microscope equipped with a 40×/100× lenses, 405, 445, 488, 515, and 561 nm lasers, an Andor FRAPPA system, an Andor Zyla sCMOS cooled camera and a Perfect Focus system
Transmission electron microscopy
Jeol JEM-1400 Plus 120kV Transmission Electron Microscope with cryo equipment and high-precision goniometer for tomographic reconstruction and for visualization of cellular ultrastructures in routine transmission electron microscopy. Equipped with a LaB6 emitter providing high resolution imaging and digital image acquisition with a Ruby 8 megapixel CCD camera. Accelerating Voltage: 40-120 kV; Magnification: Standard 800x to 800,000x, Selected Area: 2,000x to 300,000x, Low Magnification: 50x to 1,000x; Resolving Power: 3.8 Angstroms (point to point) and 2.0 Angstroms (lattice).
Analysis of molecular interactions
Biacore T100 (Biacore)
High performance system for analysis of biomolecular interactions, based on surface plasmon resonance (SPR) technology.
PC1 Photon counting spectrofluorimeter (ISS)
Fully automated for steady-state applications, corrected excitation and emission spectra, polarization (anisotropy) measurements, slow and fast kinetics, synchronous luminescence spectra, intensity measurements at fixed wavelengths, dual-wavelength excitation (emission) ratiometric measurements
BLItz System (Pall FortéBio)
Bio-Layer Interferometry (BLI) is a label-free technology for measuring biomolecular interactions. Label-free detection, real-time results, simple and fast, improves efficiency, crude sample compatibility.
Quantitative imaging
Typhoon TRIO phosphor- & fluorescence imager (GE Healthcare Life Sciences)
Variable-mode imager for radioactive, fluorescent, or chemiluminescent samples
Chemiluminescence & -fluorescence gel/membrane imager (LAS4000 Fuji)
Multipurpose CCD camera system for sensitive and quantitative imaging, chemiluminescence, trans-illumination white light and epi-illumination white light, red, green and blue fluorescence.
High performance Analysis and preparation
Multimode Microplate Reader
Fluorescence & luminescence microplate reader (Infinite M200 Pro, Tecan)
Wavelength from UV to NIR (330-600 nm to 280-850 nm) , absorbance, excitation and emission scans, photon counting luminescence, including dual color luminescence, temperature control
Particle size analyzer
Multisizer 3 COULTER COUNTER (Beckman Coulter)
Highest resolution for sizing and counting of particles via Coulter Principle (Electrical Sensing Zone Method), 0.4 µm to 1200 µm diameter 0.036 to 904.8 x 106 fL or µm³ volume
Ultracentrifuge separation
Optima MAX-XP Benchtop ultracentrifuge (Beckman)
175 μl up to 32.4 ml, speeds up to 150,000 RPM and more than 1,000,000 x g
Cell homogenizer
High-pressure Cell Disruption System TS 0.75 (Constant Systems/IUL)
Countinuously and one-shot process, pressure range up to 2700 bar (40 KPSI) for bacteria, yeast, algae, mammalian and insect cells, fungus etc.