Genetic circuits on single DNA molecules, with and without cell-like compartments

Microbiology Seminar Series

  • Date: Feb 19, 2024
  • Time: 01:15 PM (Local Time Germany)
  • Speaker: Dr. Ferdinand Greiss
  • Weizman Institute, Rehovot, Israel
  • Location: MPI for Terrestrial Microbiology
  • Room: Lecture Hall / Hybrid
  • Host: Dr. Daniel Schindler
  • Contact:

An artificial cell responds to selective pressure by point mutations on a single DNA molecule. As the long-standing vision for constructing such evolving artificial cells, we are exploring ways to engineer genetically encoded systems on single DNA molecules using cell-free gene expression: We studied decision-making with different effective noise levels in microfluidic cell-like compartments (Greiss et al., Nat Commun, 2020), we designed a pulsatile genetic circuit driven by transient RNA polymerase-mRNA-ribosome complexes on a single cell-free DNA molecule (Greiss et al., bioRxiv, 2023), and we reconstituted gene expression from single genome-scale DNA molecules in cell-like compartments.

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