Graduate Students Mini Symposium X-2023

Graduate Students Mini-Symposium

  • Date: Nov 6, 2023
  • Time: 01:15 PM (Local Time Germany)
  • Location: MPI for Terrestrial Microbiology
  • Room: Lecture Hall / Hybrid
  • Host: IMPRS
  • Contact:

Paul Alejandro Gomez Coronado - AG Erb

Sebastian Barthel - AG Erb
Prototyping a complex enzyme cascade using in vitro transcription-based biosensing

The bioproduction of special and commodity chemicals is a crucial challenge to create a sustainable industry. Yet, the optimization of biological production systems is tedious and commonly restricted by slow and expensive sample analysis. To accelerate this process, we tested the use of low-cost and well-scalable in vitro transcription (IVT)-based biosensing. We tested the concept by prototyping the CETCH cycle, a synthetic CO2-fixation cycle producing glycolate. We constructed and characterized a glycolate-responsive biosensor and explored the effects of CETCH cycle components on the IVT system. Eventually we tested CETCH cycle samples with constant and varied cofactor composition which correlated well to LC-MS quantification.

Jannik Harberding - AG Thanbichler

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