Dynamics, Feedback, and Transient Antibiotic Resistance in Single Cells

Microbiology Seminar Series

  • Date: Feb 8, 2021
  • Time: 16:00
  • Speaker: Dr. Mary Dunlop
  • Boston University, Biomedical Engineering Department
  • Location: Zoom
  • Host: Dr. Ilka Bischofs-Pfeiffer
  • Contact: ilka.bischofspfeifer@mpi-marburg.mpg.de

Cell-to-cell heterogeneity in gene expression and growth can have critical functional consequences, such as determining whether individual bacteria survive or die following antibiotic exposure. However, the timescales of the dynamics that underlie this heterogeneity are often unknown. This is a critical piece of information because slow versus fast changes in expression and growth may ultimately lead to different survival outcomes. In this talk, I will describe experiments using single-cell time-lapse microscopy to measure gene expression and growth in Escherichia coli, focusing on genes related to stress response. I will also discuss progress towards optogenetic experimental techniques for precisely perturbing gene expression dynamics in single cells.

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