Microfluidic single-cell cultivation: Concept, application and challenges

Microbiology Seminar Series

  • Date: Jan 27, 2020
  • Time: 13:15
  • Speaker: Prof. Dr. Alexander Grünberger
  • Universität Bielefeld, Technische Fakultät - AG Multiscale Bioengineering
  • Location: MPI for Terrestrial Microbiology
  • Room: Lecture hall
  • Host: Prof. Dr. Tobias Erb
  • Contact: toerb@mpi-marburg.mpg.de

Our understanding of microbial processes is still dominated by an average cell approach in which the reactor is well-stirred and all cells behave equally. In contrast, the influence of single cell response to their micro-environment, external disturbances or genetic stochasticity is still a mystery. Therefore, novel analytical methods need to be developed to understand these processes in detail [1]. In this presentation I will give an introduction into microfluidic single-cell cultivation (MSCC) and its application in Microbiology and Biotechnology. Microfluidic single-cell bioreactors [2,3] offer precisely controlled external environmental conditions and allow single-cell analysis at full spatio-temporal resolution and thus provide the opportunity to gain novel insights into fundamental and applied microbiological phenomena. Nevertheless, MSCC is still in an early phase of its development, making a critical discussion of advantages, disadvantages, existing challenges and necessary improvements indispensable [4].

[1] Grünberger, A., et al., (2014), Current Opinion in Biotechnology, 29:15-23.

[2] Grünberger, A., et al., (2012), Lab on a Chip, 12(11): 2060-2068.

[3] Grünberger, A., et al., (2015), Cytometry A, 87(12): 1101-1115.

[4] Dusny, C. & Grünberger A., (2020), Current Opinion in Biotechnology, 63:26–33.

Go to Editor View