Plant-derived regulators and their application in COMPASS optimization method

Marburger Yeast Club

  • Date: Jul 9, 2019
  • Time: 14:00
  • Speaker: Gita Naseri
  • Universität Potsdam, Junior Research Group Cell2Fab
  • Location: ZSM, Karl-von-Frisch-Str. 16
  • Room: Seminar Room
  • Host: Prof. Dr. Tobias Erb and Dr. Michal Skruzny
  • Contact: michal.skruzny@synmikro.mpi-marburg.mpg.de

In the last few decades, microorganisms such as the yeast Saccharomyces cerevisiae have been used to produce many high-value metabolites, enzymes, and pharmaceutical proteins. However, a priori knowledge about the optimal expression level of involved genes is typically not available. To achieve this, we constructed a library of 106 inducible regulators on the basis of plant transcription factor scaffolds for the tight control of the gene expression in the yeast S. cerevisiae. Next, we established a high-throughput cloning method, called COMPASS for COMbinatorial Pathway ASSembly, to balance the expression of multiple genes using plant-derived regulators. This talk will highlight our collection of plant-derived regulators, the COMPASS workflow, and its application in synthetic biology projects.

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