Production and analysis of synthetic Cascade variants

Graduate Students Mini-Symposium

  • Date: Dec 3, 2018
  • Time: 02:25 PM (Local Time Germany)
  • Speaker: Daniel Gleditzsch
  • MPI Marburg, MPRG Randau
  • Location: MPI for Terrestrial Microbiology
  • Room: Lecture hall
  • Host: IMPRS
  • Contact:

CRISPR-Cas is an adaptive immune system in bacteria and archaea that provides resistance against invading viruses. The minimal CRISPR-Cas system of Shewanella putrefaciens CN-32 utilizes a multi-subunit interference complex termed Cascade, missing small and large subunits usually required for target specificity. Crystallization reveals that structural variations substitute these proteins.

Synthetic Cascade variants can be formed by extending or shortening the backbone and complex formation can be targeted towards RNA of interest by adding a recognition sequence. The targeted RNA is wrapped in long helical filament structures which protect the RNA against degradation or can be used to silence reporter genes.

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