Insights into the inner life of living cells

Research report (imported) 2019 - Max Planck Institute for Terrestrial Microbiology

Authors
Endesfelder, Ulrike
Departments
Department of Systems and Synthetic Microbiology, Research Group Single Molecule Microbiology
Summary
Single-molecule localization microscopy offers unprecedented insights into living cells. In practice, however, many difficulties persist. By improving an important group of fluorophores, we were able to significantly reduce the damage the method causes in the imaged cells and to establish a novel, aberration-free multi-color strategy. This enables, among other things, the four-dimensional reconstruction of multi-protein-complexes such as the kinetochore in Schizosaccharomyces pombe.

For the full text, see the German version.

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