Dr. Ulrike Endesfelder (Research Group Leader)

Single molecule microbiology

Curriculum Vitae

Diploma in Physics, Bonn University (2008)
PhD (Dr. rer. nat.), Applied Laser Physics and Laser Spectroscopy, Bielefeld University (2012)
Postdoc, Biotechnology and Biophysics, Würzburg University (2012-2013)
Postdoc, Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt (2013-2014)
Project Group Leader at the Department of Systems and Synthetic Microbiology, MPI Marburg (10/2014-04/2018)
Elected member of the Junge Akademie at the BBAW and Leopoldina 2015
Research Group Leader at the Department of Systems and Synthetic Microbiology, MPI Marburg (since 05/2018)

Research Area: Single Molecule Microbiology

The group focuses on the application of techniques from the emerging field of super-resolution microscopy to study microbial cell biology. By combining physical concepts of imaging, photo-manipulation of fluorophores and quantitative read-out analyses, we aim at a single-molecule description of cellular structures and processes.

Single Molecule Localization Microscopy (SMLM) registers the position of individual fluorescent molecules. This provides microscopic images with sub-diffraction spatial resolution approaching 20 nm, and allows resolving tiny cellular structures in space. The single-molecule data can further be used for molecular counting, e.g. of protein copy numbers that may vary with respect to the cell cycle. Localization-based algorithms provide access to sub-populations and heterogeneities that are otherwise hidden through ensemble averaging, e.g. through a spatial analysis of protein distributions or the colocalization of molecules.

We apply recent experimental and analytical strategies to study cellular processes in the model organisms E. coli and S. pombe. We investigate the protein distributions and copy numbers, determine interactions and stoichiometries, and relate these findings with the cell cycle.

Nov 2017

Recent Publications

David Virant, Bjoern Traenkle, Julia Maier, Philipp D. Kaiser, Mona Bodenhoefer, Christian Schmees, Ilijana Vojnovic, Borbála Pisak-Lukáts, Ulrike Endesfelder§, Ulrich Rothbauer§ (2018)
A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging.
Nature communications, 9(1), 930; 10.1038/s41467-018-03191-2. Authors contributed equally. §Corresponding authors.
Highlighted in Tools in brief, Nat Meth 15(4), 246 (2018).

Christian Baarlink, Matthias Plessner, Alice Sherrard, Kohtaro Morita, Shinji Misu, David Virant, Eva-Maria Kleinschnitz, Robert Harniman, Dominic Alibhai, Stefan Baumeister, Kei Miyamoto, Ulrike Endesfelder, Abderrahmane Kaidi & Robert Grosse (2017)
A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.
Nature Cell Biology 19, 1389–1399; 10.1038/ncb3641.
Highlighted in News & Views, Nat Cell Biol 19, 1386–1388 (2017).

David Virant, Bartosz Turkowyd, Alexander Balinovic & Ulrike Endesfelder (2017)
Combining Primed Photoconversion and UV-Photoactivation for Aberration-Free, Live-Cell Compliant Multi-Color Single-Molecule Localization Microscopy Imaging
Int. J. Mol. Sci. 18(7), 1524; 10.3390/ijms18071524.

Bartosz Turkowyd, Alexander Balinovic, David Virant, Haruko G. Gölz Carnero, Fabienne Caldana, Marc Endesfelder, Dominique Bourgeois & Ulrike Endesfelder (2017)
A general mechanism of photoconversion of green-to-red fluorescent proteins based on blue and infrared light reduces phototoxicity in live-cell single-molecule imaging
Angew. Chem. Int. Ed. 10.1002/anie.201702870, Authors contributed equally

Bartosz Turkowyd, David Virant, Ulrike Endesfelder (2016)
From single molecules to life - microscopy at the nanoscale
Anal Bioanal Chem. doi:10.1007/s00216-016-9781-8, Authors contributed equally

Ulrike Endesfelder (2016)
Hochaufgelöste Zellbiologie
Biospektrum 22(2). doi: 10.1007/s12268-016-0675-2

Complete publication list

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