Equipment Imaging

Nikon Ti-E inverted widefield fluorescence microscope 
Xcite exacte ultrastable fluorescence light source. 515 nm, 532 nm, and 594 nm laser lines (dedicated to acceptor photobleachhing FRET). 
Perfect Focus System. NIS elements and Nikon Jobs software for automatic image acquisition and analysis. Andor iXON897 EMCCD camera.
Incubator (OkoLab)

Zeiss LSM880 Laser Scanning Confocal Microscope
Laser lines (CW): 440nm, 457 nm, 488 nm, 514 nm, 543 nm, 594 nm, 633 nm. Laser lines (pulsed): 440 nm, 488-640 nm (tunable laser).
Spectral detector: 32 channels GaAsP PMTs and 2 MA-PMTs.
Definite Focus. Fluorescence lifetime imaging microscopy module (ISS).
Incubator (PeCon).

 

Applications

  • Confocal imaging
  • Automatic imaging in multi-well plates
  • Spectral Imaging
  • Acceptor photobleaching FRET
  • Ratiometric FRET
  • Fluorescence lifetime imaging microscopy
  • Fluorescence correlation and cross-correlation spectroscopy for measurement of protein diffusion and protein-protein interactions
  • Photon Counting Histogram
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