Publications

2026
Preprint
Werland, L.; Rinn, J.; Malengo, G.; Kholeif, K.; Glatter, T.; Colin, R.; Sourjik, V.; Yuan, J.: Antagonistic small proteins enable magnesium-dependent tuning of two-component signaling. bioRxiv: the preprint server for biology, 2026.05.06.723403 (2026)
Zeitschriftenartikel
Wang, L.; Malengo, G.; Sanches-Medeiros, A.; Chen, X.; Pietsch, J. M. J.; Teteneva, N. A.; Sierra González, S.; Hammond, M. C.; Sourjik, V.: Toolbox of FRET-based c-di-GMP biosensors and its FRET-To-Sort application for genome-wide mapping of c-di-GMP regulation. Nature Communications 17 (1), 2955 (2026)
2025
Zeitschriftenartikel
Chen, H.; Malengo, G.; Wang, L.; Vogler, O.; Renner, M.; Glatter, T.; Paczia, N.; Unterweger, D.; Sourjik, V.; Diepold, A.: Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis. Communications Biology 8 (1), 1236 (2025)
Zeitschriftenartikel
Pintor, K. L.; Balinovic, A.; Malengo, G.; Klepin, C.; Fleck, M.; Glatter, T.; Endesfelder, U.; Diepold, A.: In situ analysis of type III secretion chaperone proteins indicates a cytosolic handover of virulence effectors. FEMS Microbes 6, xtaf010 (2025)
2023
Zeitschriftenartikel
Jiang, S.; Steup, L. C.; Kippnich, C.; Lazaridi, S.; Malengo, G.; Lemmin, T.; Yuan, J.: The inhibitory mechanism of a small protein reveals its role in antimicrobial peptide sensing. Proceedings of the National Academy of Sciences of the United States of America 120 (41), e2309607120 (2023)
Preprint
Jiang, S.; Steup, L. C.; Kippnich, C.; Lazaridi, S.; Malengo, G.; Lemmin, T.; Yuan, J.: The inhibitory mechanism of a small protein reveals its role in antimicrobial peptide sensing. bioRxiv: the preprint server for biology, 2022.12.22.521624 (2023)
2022
Zeitschriftenartikel
Bellotto, N.; Agudo-Canalejo, J.; Colin, R.; Golestanian, R.; Malengo, G.; Sourjik, V.: Dependence of diffusion in Escherichia coli cytoplasm on protein size, environmental conditions and cell growth. eLife 11, e82654 (2022)
Preprint
Bellotto, N.; Agudo-Canalejo, J.; Colin, R.; Golestanian, R.; Malengo, G.; Sourjik, V.: Dependence of diffusion in Escherichia coli cytoplasm on protein size, environmental conditions and cell growth. bioRxiv: the preprint server for biology, 2022.02.17.480843 (2022)
2021
Zeitschriftenartikel
Abella, M.; Andruck, L.; Malengo, G.; Skruzny, M.: Actin-generated force applied during endocytosis measured by Sla2-based FRET tension sensors. Developmental Cell 56 (17), S. 2419 - 2426.e4 (2021)
2020
Zeitschriftenartikel
Yadavalli, S. S.; Goh, T.; Carey, J. N.; Malengo, G.; Vellappan, S.; Nickels, B. E.; Sourjik, V.; Goulian, M.; Yuan, J.: Functional Determinants of a Small Protein Controlling a Broadly Conserved Bacterial Sensor Kinase. JOURNAL OF BACTERIOLOGY 202 (16), e00305-20 (2020)
Zeitschriftenartikel
Skruzny, M.; Pohl, E.; Gnoth, S.; Malengo, G.; Sourjik, V.: The protein architecture of the endocytic coat analyzed by FRET microscopy. MOLECULAR SYSTEMS BIOLOGY 16 (5), e9009 (2020)
Zeitschriftenartikel
Babel, H.; Naranjo-Meneses, P.; Trauth, S.; Schulmeister, S.; Malengo, G.; Sourjik, V.; Bischofs, I. B.: Ratiometric population sensing by a pump-probe signaling system in Bacillus subtilis. NATURE COMMUNICATIONS 11 (1), 1176 (2020)
2016
Zeitschriftenartikel
Gryaznova, Y.; Caydasi, A.K.; Malengo, G.; Sourjik, V.; Pereira, G.: A FRET-based study reveals site-specific regulation of spindle position checkpoint proteins at yeast centrosomes. eLife 5, e14029 (2016)
2013
Zeitschriftenartikel
Wille, T.; Wagner, C.; Mittelstadt, W.; Blank, K.; Sommer, E.; Malengo, G.; Dohler, D.; Lange, A.; Sourjik, V.; Hensel, M. et al.; Gerlach, R. G.: SiiA and SiiB are novel type I secretion system subunits controlling SPI4-mediated adhesion of Salmonella enterica. Cell Microbiol 16 (2), S. 161 - 78 (2013)
2008
Zeitschriftenartikel
Malengo, G.; Andolfo, A.; Sidenius, N.; Gratton, E.; Zamai, M.; Caiolfa, V. R.: Fluorescence correlation spectroscopy and photon counting histogram on membrane proteins: functional dynamics of the glycosylphosphatidylinositol-anchored urokinase plasminogen activator receptor. J Biomed Opt 13 (3), S. 031215 (2008)
2007
Zeitschriftenartikel
Caiolfa, V. R.; Zamai, M.; Malengo, G.; Andolfo, A.; Madsen, C. D.; Sutin, J.; Digman, M. A.; Gratton, E.; Blasi, F.; Sidenius, N.: Monomer dimer dynamics and distribution of GPI-anchored uPAR are determined by cell surface protein assemblies. J Cell Biol 179 (5), S. 1067 - 82 (2007)
2004
Konferenzband
Scanning algorithms in high-sensitivity two-photon excitation microscopy for single-molecule investigations (Biomedical Optics 2004, 5323). SPIE (2004), PWB S.

Babel, H., Naranjo-Menenes, P., Trauth, S., Schulmeister, S., Malengo, G., Sourjik, V., Bischofs, I.B. (2020)
Ratiometric population sensing by a pump-probe signaling system in Bacillus subtilis. Nat Commun. 11(1): 1176, doi: 10.1038/s41467-020-14840-w

Skruzny, M., Pohl, E., Gnoth, S., Malengo, G., Sourjik, V. (2020)
The protein architecture of the endocytic coat analyzed by FRET microscopy. Mol Syst Biol. 16(5): e9009, doi: 10.15252/msb.20199009

Schramm T, Lempp M, Beuter D, González Sierra S., Glatter T, Link H. (2020)
High-throughput enrichment of temperature-sensitive argininosuccinate synthetase for two-stage citrulline production in E. coli. Metab Eng. 60:14-24. doi: 0.1016/j.ymben.2020.03.004

Yadavalli, S., Goh, T., Carey, J., Malengo, G., Nickels, B.,Sourjik, V., Goulian, M., Juan, Y. (2020)
Functional determinants of a small protein controlling a broadly conserved bacterial sensor kinase. J. Bacteriol JB.00305-20, doi: 10.1128/JB.00305-20

Muhr E., Leicht O., González Sierra S., Thanbichler M. and Heider J (2016)
A Fluorescent Bioreporter for Acetophenone and 1-Phenylethanol derived from a Specifically Induced Catabolic Operon. Front Microbiol. 2016 Jan 28;6:1561. doi: 10.3389/fmicb.2015.01561

Gryaznova Y., Koca Caydasi A., Malengo G., Sourjik V., Pereira G. (2016) 
A FRET-based study reveals site-specific regulation of spindle position checkpoint proteins at yeast centrosomes. Elife. May 9;5. pii: e14029, doi: 10.7554/eLife.14029

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