Graduate Students Mini Symposium VI 2021

16:00 Francisco Javier Arriaza Gallardo (RG Shima)

Title and abstract: TBA

16:30 Tolganay Kabdullayeva (RG Becker)

In vitro and in vivo long-term digital information storage in DNA

The MOlecular Storage for Long-term Archiving (MOSLA) research cluster investigates the application of molecular memories for long-term digital data storage.

In the frames of our collaboration with the Computer Science department, we explore the possibility of digital information storage in DNA molecules under in vitro and in vivo conditions.

In vitro approach utilizes the oligonucleotide libraries equipped with adapters for data replication and readout via PCR and NGS. In comparison, for the in vivo method we integrate the digital information in the genomes of vegetative cells of Sinorhizobium meliloti and endospores of Bacillus subtilis, which are then subject to stability tests under various environmental conditions to study the difference between encoding algorithms and genome architectures.

17:00 Erick Arroyo Perez (RG Ringgaard)

A novel protein regulating flagellum formation in Vibrio parahaemolyticus

Many bacterial species rely on flagellum mediated motility to explore their surroundings. The number and localization of flagella within a bacterium are properties intrinsic to each bacterial species. The proper positioning of the flagellum is necessary for the bacterial cell to be able to swim in its environment. Vibrio bacteria produce a single polar flagellum and its architecture and positioning is coordinated by an unclear mechanism involving two nucleotide hydrolases, FlhF and FlhG. We have identified a new protein that is essential for the localization and function of FlhF, and is required for flagellum formation and swimming motility.

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