Plant-derived regulators and their application in COMPASS optimization method

In the last few decades, microorganisms such as the yeast Saccharomyces cerevisiae have been used to produce many high-value metabolites, enzymes, and pharmaceutical proteins. However, a priori knowledge about the optimal expression level of involved genes is typically not available. To achieve this, we constructed a library of 106 inducible regulators on the basis of plant transcription factor scaffolds for the tight control of the gene expression in the yeast S. cerevisiae. Next, we established a high-throughput cloning method, called COMPASS for COMbinatorial Pathway ASSembly, to balance the expression of multiple genes using plant-derived regulators. This talk will highlight our collection of plant-derived regulators, the COMPASS workflow, and its application in synthetic biology projects.