Determination of the substrate recognition by fumarate adding enzymes

Graduate Students Mini-Symposium

  • Date: Aug 13, 2018
  • Time: 01:15 PM (Local Time Germany)
  • Speaker: Iryna Salii
  • Uni Marburg, AG Heider
  • Location: MPI for Terrestrial Microbiology
  • Room: Lecture hall
  • Host: IMPRS
  • Contact:

The first step of anaerobic degradation of aromatic hydrocarbons toluene and 2-methylnaphthalene are catalyzed by the glycyl radical enzymes benzylsuccinate synthase (BSS) and newly discovered naphthyl-2-methylsuccinate synthase (NMS) which catalyze the radical addition of toluene and 2-methylnaphthalene to fumarate, producing benzylsuccinate and naphthyl-2-methylsuccinate. We developed the first recombinant overproduction system of active benzylsuccinate synthase and naphthyl-2-methylsuccinate synthase. Also, we present initial data on the effects of some constructed active-site mutants in the alpha subunit of BSS and initially characterize substrate spectrum of NMS. This study provides new insight on the reaction mechanism and substrate recognition of both enzymes.

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