Selected publications 2000 - 2014

Brock, M., Fischer, R., Linder, D. & Buckel, W. (2000) 
Methylcitrate synthase from Aspergillus nidulans: Implications for propionate as an antifungal agent. Mol. Microbiol. 35, 961-973.

Dickert, S., Pierik, A., Linder, D., & Buckel, W. (2000)
The involvement of coenzyme A esters in the dehydratation of (R)-phenyllactate to (E)-cinnamate by Clostridium sporogenes Eur. J. Biochem. 267, 3874-3884.

Gerhardt, A., Çinkaya, I., Linder, D., Huisman, G. & Buckel, W. (2000)
Fermentation of 4-aminobutyrate by Clostridium aminobutyricum: Cloning of two genes involved in the formation and dehydration of 4-hydroxybutyryl-CoA. Arch. Microbiol. 174, 189-199.

Buckel, W. (2001a)
Sodium ion translocating decarboxylases. Minireview, BBA-Bioenergetics, Biochim. Biophys. Acta 1505, 15-27.

Hans, M. & Buckel, W. (2000)
Purification of recombinant component A of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans using Strep-Tactin affinity-chromatography. Biotech. International 12, 12.

Locher, K. P., Hans, M., Yeh, A. P., Buckel, W. & Rees, D. C. (2001)
Crystal structure of he Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A.  J. Mol. Biol. 307, 297-308.

Brock, M., Darley, D., Textor, S. & Buckel, W. (2001)
2-Methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans: Characterisation and comparison of both enzymes. Eur. J. Biochem. 268, 3577-3586.

Buckel, W. (2001b)
Unusual enzymes involved in five pathways of glutamate fermentation. Applied Microbiol. Biotechnol. 57, 263-273

Hans, M., Bill, E., Cirpus, I., Pierik, A. J., Hetzel, M., Alber, D. & Buckel, W. (2002)
Adenosine triphosphate-induced electron transfer in 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. Biochemistry 41, 5873-5882.

Brock, M., Maerker, C., Schütz, A., Völker, U., & Buckel, W (2002)
Oxidation of propionate to pyruvate in Escherichia coli: Involvement of methylcitrate dehydratase, PrpD, and aconitase, AcnB. Eur. J. Biochem. 269, 6184-6194.

Buckel, W., Selmer, T., Selofonova, O.V., Gokarn, R.R., Gort, S.J. & Jessen, H.  (2002)
3-Hydroxypropanoic acid and other organic compounds. World Patent WO0242418

Hetzel, M., Brock, M., Selmer, T., Pierik, A. J., Golding, B. T., & Buckel, W. (2003)
Acryloyl-CoA reductase from Clostridium propionicum: An enzyme complex of propionyl-CoA dehydrogenase and electron-transferring flavoprotein. Eur. J. Biochem. 270, 902-910.

Thamer, W., Cirpus, I., Hans, M., Pierik, A. J., Selmer, T., Bill, E., Linder, D. & Buckel, W. (2003)
A two [4Fe-4S] cluster containing ferredoxin as an alternative electron donor for 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans. Arch. Microbiol. 179, 197-204.

Smith, D. M., Buckel, W. & Zipse, H. (2003)
Deprotonierung von Enoxy-Radikalen: theoretische Bestätigung eines 50 Jahre alten Mechanismusvorschlags, Angew. Chem. 115, 1911-1915. Deprotonation of enoxy radicals: Theoretical Validation of a 50-Year-Old Mechanistic Proposal, Angew. Chem. Int. Ed. Engl. 42, 1867-1870.

Wendt, K. S., Schall, I., Huber, R., Buckel, W., & Jacob, U. (2003)
Crystal structure of the carboxyltransferase subunit of the bacterial sodium ion pump glutaconyl-coenzyme A decarboxylase. EMBO J. 22, 3493-3502.

Brock, M. & Buckel, W. (2004)
On the mechanism of action of the antifungal agent propionate: Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans. Eur. J. Biochem. 271, 3227-3241.

Buckel, W., Hetzel, M. & Kim, J. (2004)
ATP-driven electron transfer in enzymatic radical reactions. Curr. Opin. Chem. Biol. 8, 462-467.

Martins, B. M., Dobbek, H., Çinkaya. I., Buckel, W. & Messerschmidt, A. (2004)
Crystal structure of 4-hydroxybutyryl-CoA dehydratase: Radical catalysis involving a [4Fe-4S] cluster and flavin. Proc. Natl. Acad. Sci. USA 101, 15645-15649.

Kim, J., Darley, D. & Buckel, W. (2005)
2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile, FEBS Journal 272, 550-561.

Buckel, W. (2005)
Special clostridial enzymes and fermentation pathways. In Clostrida (P. Dürre, ed), CRC Press, Taylor & Francis Group, Atlanta, GA, USA, pp. 177-220.

Pierik, A. J., Ciceri, D., Lopez, R. F., Kroll, F., Bröker, G., Beatrix, B., Buckel, W. & Golding, B. T. (2005)
Searching for intermediates in the carbon skeleton rearrangement of 2-methyleneglutarate to (R)-3-methylitaconate catalysed by coenzyme B12-dependent 2-methyleneglutarate mutase from Eubacterium barkeri, Biochemistry 44, 10541-10551.

Buckel, W., Kratky, C. & Golding, B. T. (2005)
Stabilisation of methylene radicals by cob(II)alamin in coenzyme B12-dependent mutases, Chem. Eur. J. 12, 352-362

Boiangiu, C. D., Jayamani, E., Brügel, D., Herrmann, G., Forzi, L., Hedderich, R., Vgenopoulou, I., Kim, J., Pierik, A. J., Steuber, J. & Buckel, W. (2005)
Sodium ion pumps and hydrogen production in glutamate fermenting anaerobic bacteria, J. Mol. Microbiol. Biotechnol. 10, 105-119.

Buckel, W. & Golding, B. T. (2006)
Radical enzymes in anaerobes. Ann. Rev. Microbiol., 60, 27-49.

Mack, M., Liesert, M., Zschocke, J., Peters, V., Linder, D. & Buckel, W. (2006)
3-Methylglutaconyl-CoA hydratase from Acinetobacter sp. Arch. Microbiol., 185, 297–306.

Kim, J., Lu, Y., & Buckel, W. (2007)
ATP- and redox-induced conformational changes in the activator of the radical enzyme 2-hydroxyisocaproyl-CoA dehydratase. C. R. Chimie 10, 742-747.

Imkamp, F., Biegel, E., Jayamani, E., Buckel, W. & Müller, V. (2007)
Dissection of the caffeate respiratory chain in the acetogen Acetobacterium woodii: identification of an Rnf-type NADH dehydrogenase as potential coupling site, J. Bacteriol. 189, 8145-8153.

Berg, I. A., Kockelkorn, D., Buckel, W., and Fuchs, G. (2007)
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea, Science 318, 1782-1786.

Herrmann, G., Jayamani, E., Mai, G. & Buckel, W. (2008)
Energy conservation via electron transferring flavoprotein (Etf) in anaerobic bacteria. J. Bacteriol. 190, 784-791.

Li, F., Hinderberger, J., Seedorf, H., Zhang, J., Buckel, W. & Thauer, R. K. (2008)
Coupled ferredoxin- and crotonyl-CoA reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/ETF complex from Clostridium kluyveri. J. Bacteriol. 190, 843-850.

Seedorf, H., Fricke, W. F., Veith, B., Brüggemann, H., Liesegang, H, Strittmatter, A., Miethke, M., Buckel, W., Hinderberger, J., Li, F., Hagemeier, C., Thauer, R. K., & Gottschalk, G. (2008)
The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features. Proc. Natl. Acd. Sci. USA 105, 2128-2133.

Kim, J., Darley, D., Buckel, W. & Pierik, A. J. (2008)
An allylic ketyl radical intermediate in clostridial amino-acid fermentation, Nature 452, 239-242.

Hans, M., Buckel, W. & Bill, E. (2008)
Spectroscopic evidence for an all-ferrous [4Fe-4S]0 cluster in the super-reduced activator of 2-hydroxyglutaryl-CoA dehydratase from Acidaminococcus fermentans, J. Inorg. Biol. Chem. 13, 563-574.

Friedrich, P., Darley, D., Golding, B. T. & Buckel, W. (2008)
Der stereochemische Verlauf der enzymatischen Wassereliminierung von 4-Hydroxybutyryl-Coenzym A zu Crotonyl-Coenzym A. Angew. Chem. 120, 3298-3301. The complete stereochemistry of the enzymatic dehydration of 4-hydroxybutyryl-coenzyme A to crotonyl-coenzyme A. Angew. Chem. Int. Ed. Engl. 47, 3254-3257.

Thauer, R. K., Kaster, A. K., Seedorf, H., Buckel, W., & Hedderich, R. (2008)
Methanogenic archaea: ecologically relevant differences in energy conservation. Nat. Rev. Microbiol. 6, 579-591

Berg, I., Kockelkorn, D., Buckel, W. & Fuchs, G. (2008)
Response to the comment on "A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea." Science 321, 342c

Buckel, W. (2009)
Katalyse durch Rezyklierung von Radikalen oder Elektronen. Angew. Chem. 121, 6911-6920. Radical and electron recycling in catalysis. Angew. Chem. Int. Edn. 48, 6779-6787.

Kim, J., Pierik, A. J. & Buckel, W. (2010)
A complex of 2-hydroxyisocaproyl-coenzyme A dehydratase and its activator from Clostridium difficile stabilized by aluminium tetrafluoride-adenosine diphosphate. ChemPhysChem, 11, 1307-1312.

Parthasarathy, A., Buckel, W. & Smith, D. M. (2010)
On the thermodynamic equilibrium between (R)-2-hydroxyacyl-CoA and 2-enoyl-CoA. FEBS J. 277, 1738-1746.

{Djurdjevic, 2010 #316}Djurdjevic, I., Zelder, O. & Buckel, W. (2011)
Production of glutaconic acid in a recombinant Escherichia coli strain, Appl. Environ. Microbiol. 77, 320–322. 

Knauer, S. H., Buckel, W. & Dobbek, H. (2011)
Structural Basis for Reductive Radical Formation and Electron Recycling in (R)-2-Hydroxyisocaproyl-CoA Dehydratase., J. Amer. Chem. Soc., 133, 4342-4347.

Parthasarathy, A., Pierik, A. J., Kahnt, J., Zelder, O. & Buckel, W. (2011)
Substrate specificity of 2-hydroxyglutaryl-CoA dehydratase from Clostridium symbiosum: towards a bio-based production of adipic acid, Biochemistry 50, 3540-3550. Erratum in: Biochemistry. 50, 4392 (2011).

Martins, B. M., Blaser, M., Feliks, M., Ullmann, G. M., Buckel, W. & Selmer, T. (2011)
Structural basis for a Kolbe-type decarboxylation catalyzed by a glycyl radical enzyme, J. Amer. Chem. Soc. 133,14666-14674.

Jarling, R., Sadeghi, M., Drozdowska, M., Lahme, S., Buckel, W., Rabus, R., Widdel, F., Golding, B.T. and Wilkes, H. (2012)
Stereochemical Investigations Reveal the Mechanism of the Bacterial Activation of n-Alkanes without Oxygen. Angew. Chem. Int. Ed. 51, 1334–1338. Stereochemische Untersuchungen enthüllen den Mechanismus der sauerstofffreien Aktivierung von n-Alkanen durch Bakterien. Angew. Chem. 124, 1362–1366.

Buckel, W., Zhang, J., Friedrich, P., Parthasarathy, A., Li, H., Djurdjevic, I., Dobbek, H. & Martins, B. M. (2012)
Enzyme catalyzed radical dehydrations of hydroxy acids, Biochem. Biophys. Acta, 1824, 1278-1290.

Friedrich, P., Baisch, F., Harrington, R., Lyatuu, F. E., Zhou, K., Zelder, F., McFarlane, W., Buckel, W. & Golding, B. T. (2012)
Experimental Study of Hydrogen Bonding Potentially Stabilizing the 5'-Deoxyadenosyl Radical from Coenzyme B12. Chemistry 18, 16114-16122.

Knauer, S. H., Buckel, W. & Dobbek, H. (2012)
On the ATP-dependent Activation of the Radical Enzyme (R)-2-Hydroxyisocaproyl-CoA Dehydratase. Biochemistry 51, 6609-6622.

Buckel, W. & Thauer, R. K. (2013)
Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation Biochem. Biophys. Acta 1827, 94–113.

Kandasamy, V., Vaidyanathan, H., Djurdjevic, I., Jayamani, E., Ramachandran, K. B., Buckel, W., Jayaraman, G. & Ramalingam, S. (2013)
Engineering Escherichia coli with acrylate pathway genes for propionic acid synthesis and its impact on mixed-acid fermentation. Appl Microbiol Biotechnol, 97, 1191-1200.

Kim, M., Le, H., McInerney, M. & Buckel, W. (2013)
Re-Citrate synthase from Syntrophus aciditrophicus, J Bacteriol. 195, 1689-96.

Chowdhury, N. P., Mowafy, A. M., Demmer, J. K., Upadhyay, V., Koelzer, S., Jayamanini, E., Kahnt, J., Hornung, M., Demmer, U., Ermler, U. & Buckel, W. (2014)
Studies on the mechanism of electron bifurcation catalyzed by electron transferring flavoprotein (Etf) and butyryl-CoA dehydrogenase (Bcd) of Acidaminococcus fermentans, J. Biol. Chem. 289, 5145-5157.

Buckel, W. (2014)
Wie Anaerobier Ferredoxin reduzieren. BIOspektrum 20, 738-740.


Publications since 2015

Zhang, J., Friedrich, P., Pierik, A., Martins, B. M. & Buckel, W. (2015)
Substrate induced radical formation in 4-hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum. Appl. Environ. Microbiol. 81, 1071-1084.

Chowdhury, N. P., Kahnt, J. & Buckel, W. (2015)
Reduction of ferredoxin or oxygen by flavin-based electron bifurcation in Megasphaera elsdenii. FEBS J. 282, 3149–3160.

Kim, M., Le, H. M., Xie, X., Feng, X., Tang, Y. J., Mouttaki, H., McInerney, M. J. & Buckel, W. (2015)
Two pathways for glutamate biosynthesis in the syntrophic bacterium, Syntrophus aciditrophicus. Appl. Environ. Microbiol. 81, 8434-8444.

Buckel, W. (2015)
“Life’s engines: How Microbes Made the Earth Habitable” von Paul Falkowski. Buchrezension, BIOspektrum 21 (7), 795-796.

Schönheit, P., Buckel, W. & Martin, W. F. (2016)
On the origin of heterotrophy. Trends in Microbiology. 24, 12-25. doi: 10.1016/j.tim.2015.10.003

Geiser, E., Przybilla, S. K., Friedrich, A., Buckel, W., Wierckx, N., Blank, L. M. & Bölker, M. (2016)
A novel biosynthetic pathway for microbial itaconic acid production via the unusual intermediate trans-aconitate. Microb. Biotechnol. 9, 116-126. doi: 10.1111/1751-7915.12329. Epub 2015 Dec 7.2015

Selvaraj, B., Buckel, W., Golding, B. T., Ullmann, G. M. & Martins, B. M. (2016)
Structure and function of 4-hydroxyphenylacetate decarboxylase and its cognate activating enzyme. J. Mol. Microbiol. Biotechnol. 26, 76–91.

Wilkes, H., Buckel, W., Golding, B. T. & Rabus, R. (2016)
Metabolism of Hydrocarbons in n-Alkane-Utilizing Anaerobic Bacteria. J. Mol. Microbiol. Biotechnol. 26, 138–151.

Heider, J., Szaleniec, M., Martins, B. M., Seyhan, D., Buckel, W. & Golding, B. T. (2016)
Structure and function of benzylsuccinate synthase and related fumarate-adding glycyl radical enzymes. J. Mol. Microbiol. Biotechnol. 26, 29–44.

Boll, M., Kung, J., Ermler, U., Martins, B. M. & Buckel, W. (2016)
Cyclohexane carboxylate fermentation in Syntrophus aciditrophicus. J. Mol. Microbiol. Biotechnol. 26, 165–179.

Rabus, R., Boll, M., Heider, J., Meckenstock, R. U., Buckel, W., Einsle, O., Ermler, U., Golding, B. T., Gunsalus, R., Kroneck, P. M. H., Krüger, M., Lueders, T., Martins, B. M., Musat, F., Richnow, H., Schink, B., Seifert, J., Szaleniec, M., Treude, T., Ullmann, G. M., Vogt, C., von Bergen, M. & Wilkes, H. (2016)
Anaerobic Microbial Degradation of Hydrocarbons: From Enzymatic Reactions to the Environment. J. Mol. Microbiol. Biotechnol. 26, 5–28. DOI: 10.1159/000443997

Angenent L. T., Richter H., Buckel W., Spirito C. M., Steinbusch K. J. J., Plugge C. M., Strik D. P. B. T. B., Grootscholten T. I. M., Buisman C. J. N. & Hamelers H. V. M. (2016).
Chain elongation with reactor microbiomes: open-culture biotechnology to produce biochemicals. Environmental Science & Technology. 50, 2796-2810.

Poehlein, A., Schlien, K., Chowdhury, N., Gottschalk, G., Buckel, W. & Daniel, R. (2016)
Complete genome sequence of the amino acid-fermenting Clostridium propionicum X2 (DSM 1682) genomeA00294-16

Chowdhury, N. P., Klomann, K., Seubert, A. & Buckel, W. (2016)
Reduction of flavodoxin by electron bifurcation and sodium ion-dependent re-oxidation by NAD+ catalyzed by ferredoxin:NAD+ reductase (Rnf) J. Biol. Chem. Apr 5. pii: jbc.M116.726299. [Epub ahead of print] PMID: 27048649


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