Publications

2024
Preprint
Liu, Y.; Karmakar, R.; Steinchen, W.; Mukherjee, S.; Bange, G.; Schäfer, L. V.; Thanbichler, M.: Membrane binding properties of the cytoskeletal protein bactofilin. bioRxiv: the preprint server for biology, 2024.06.14.599034 (2024)
Journal Article
Sanchez-Londono, M.; Rust, S.; Hernandez-Tamayo, R.; Gomes-Filho, J. V.; Thanbichler, M.; Randau, L.: Visualization of Type IV-A1 CRISPR-mediated repression of gene expression and plasmid replication. bioRxiv: the preprint server for biology, 2024.06.12.598411 (2024)
Journal Article
Körner, D.; Schäfer, N. M.; Lagares Jr., A.; Birmes, L.; Oehlmann, N. N.; Addison, H. G.; Pöhl, S.; Thanbichler, M.; Rebelein, J. G.; Petersen, J. et al.; Becker, A.: Modular low-copy-number plasmid vectors for Rhodobacterales with extended host range in Alphaproteobacteria. ACS Synthetic Biology 13 (5), pp. 1537 - 1548 (2024)
Journal Article
Pöhl, S.; Osorio Valeriano, M.; Cserti, E.; Harberding, J.; Hernandez Tamayo, R.; Biboy, J.; Sobetzko, P.; Vollmer, W.; Graumann, P. L.; Thanbichler, M.: A dynamic bactofilin cytoskeleton cooperates with an M23 endopeptidase to control bacterial morphogenesis. eLife 12, RP86577 (2024)
Journal Article
Jakob, S.; Steinchen, W.; Hanßmann, J.; Rosum, J.; Langenfeld, K.; Osorio-Valeriano, M.; Steube, N.; Giammarinaro, P. I.; Hochberg, G. K. A.; Glatter, T. et al.; Bange, G.; Diepold, A.; Thanbichler, M.: The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression. Nature Communications 15 (1), 318 (2024)
2023
Preprint
Billini, M.; Hoffmann, T.; Kuhn, J.; Bremer, E.; Thanbichler, M.: The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus. bioRxiv: the preprint server for biology, 2023.10.07.561339 (2023)
Journal Article
Kremer, K.; Meier, D.; Theis, L.; Miller, S.; Rost-Nasshan, A.; Naing, Y. T.; Zarzycki, J.; Paczia, N.; Serrania, J.; Blumenkamp, P. et al.; Goesmann, A.; Becker, A.; Thanbichler, M.; Hochberg, G. K. A.; Carter, M. S.; Erb, T. J.: Functional degeneracy in Paracoccus denitrificans Pd1222 is coordinated via RamB, which links expression of the Glyoxylate cycle to activity of the Ethylmalonyl-CoA pathway. Applied and Environmental Microbiology 89 (7), e0023823 (2023)
Journal Article
Connolley, L.; Schnabel, L.; Thanbichler, M.; Murray, S. M.: Partition complex structure can arise from sliding and bridging of ParB dimers. Nature Communications 14 (1), 4567 (2023)
Journal Article
Izquierdo Martinez, A.; Billini, M.; Miguel-Ruano, V.; Hernández-Tamayo, R.; Richter, P.; Biboy, J.; Batuecas, M. T.; Glatter, T.; Vollmer, W.; Graumann, P. L. et al.; Hermoso, J. A.; Thanbichler, M.: DipM controls multiple autolysins and mediates a regulatory feedback loop promoting cell constriction in Caulobacter crescentus. Nature Communications 14 (1), 4095 (2023)
Preprint
Jakob, S.; Steinchen, W.; Hanssmann, J.; Rosum, J.; Osorio-Valeriano, M.; Giammarinaro, P. I.; Bange, G.; Thanbichler, M.: The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression. bioRxiv: the preprint server for biology, 2023.06.01.543266 (2023)
Preprint
Pöhl, S.; Osorio-Valeriano, M.; Cserti, E.; Harberding, J.; Hernández-Tamayo, R.; Biboy, J.; Sobetzko, P.; Vollmer, W.; Graumann, P. L.; Thanbichler, M.: A dynamic bactofilin cytoskeleton cooperates with an M23 endopeptidase to control bacterial morphogenesis. bioRxiv: the preprint server for biology, 2023.02.27.530196 (2023)
2022
Journal Article
Corrales-Guerrero, L.; Steinchen, W.; Ramm, B.; Mücksch, J.; Rosum, J.; Refes, Y.; Heimerl, T.; Bange, G.; Schwille, P.; Thanbichler, M.: MipZ caps the plus-end of FtsZ polymers to promote their rapid disassembly. Proceedings of the National Academy of Sciences of the United States of America 119 (50), e2208227119 (2022)
Preprint
Izquierdo-Martinez, A.; Miguel-Ruano, V.; Hernández-Tamayo, R.; Biboy, J.; Batuecas, M. T.; Billini, M.; Glatter, T.; Vollmer, W.; Graumann, P. L.; Hermoso, J. A. et al.; Thanbichler, M.: DipM controls multiple autolysins and mediates two regulatory feedback loops promoting cell constriction in C. crescentus. bioRxiv: the preprint server for biology, 2022.09.10.507391 (2022)
Preprint
Connolley, L.; Schnabel, L.; Thanbichler, M.; Murray, S. M.: Partition complex structure can arise from sliding and bridging of ParB dimers. bioRxiv: the preprint server for biology, 2022.12.01.518708 (2022)
2021
Journal Article
Osorio-Valeriano, M.; Altegoer, F.; Das, C. K.; Steinchen, W.; Panis, G.; Connolley, L.; Giacomelli, G.; Feddersen, H.; Corrales-Guerrero, L.; Giammarinaro, P. I. et al.; Hanssmann, J.; Bramkamp, M.; Viollier, P. H.; Murray, S.; Schafer, L. V.; Bange, G.; Thanbichler, M.: The CTPase activity of ParB determines the size and dynamics of prokaryotic DNA partition complexes. Molecular Cell 81 (19), pp. 3992 - 4007 e10 (2021)
Journal Article
Messelink, J. J. B.; van Teeseling, M. C. F.; Janssen, J.; Thanbichler, M.; Broedersz, C. P.: Learning the distribution of single-cell chromosome conformations in bacteria reveals emergent order across genomic scales. Nature Communications 12 (1), 1963 (2021)
2020
Journal Article
van Teeseling, M. C. F.; Thanbichler, M.: Generating asymmetry in a changing environment: cell cycle regulation in dimorphic alphaproteobacteria. BIOLOGICAL CHEMISTRY 401 (12), pp. 1349 - 1363 (2020)
Journal Article
Hartmann, R.; van Teeseling, M. C. F.; Thanbichler, M.; Drescher, K.: BacStalk: A comprehensive and interactive image analysis software tool for bacterial cell biology. MOLECULAR MICROBIOLOGY 114 (1), pp. 140 - 150 (2020)
Journal Article
Mueller-Esparza, H.; Osorio-Valeriano, M.; Steube, N.; Thanbichler, M.; Randau, L.: Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes. FRONTIERS IN MOLECULAR BIOSCIENCES 7, 98 (2020)
Journal Article
Corrales-Guerrero, L.; He, B.; Refes, Y.; Panis, G.; Bange, G.; Viollier, P. H.; Steinchen, W.; Thanbichler, M.: Molecular architecture of the DNA-binding sites of the P-loop MipZ and ParA from Caulobacter crescentus. NUCLEIC ACIDS RESEARCH 48 (9), pp. 4769 - 4779 (2020)
Journal Article
El Najjar, N.; van Teeseling, M. C. F.; Mayer, B.; Hermann, S.; Thanbichler, M.; Graumann, P. L.: Bacterial cell growth is arrested by violet and blue, but not yellow light excitation during fluorescence microscopy. BMC MOLECULAR AND CELL BIOLOGY 21 (1), 35 (2020)
Journal Article
Leicht, O.; van Teeseling, M. C. F.; Panis, G.; Reif, C.; Wendt, H.; Viollier, P. H.; Thanbichler, M.; Casadesus, J.: Integrative and quantitative view of the CtrA regulatory network in a stalked budding bacterium. PLOS GENETICS 16 (4), e1008724 (2020)
2019
Journal Article
Toro-Nahuelpan, M.; Corrales-Guerrero, L.; Zwiener, T.; Osorio-Valeriano, M.; Mueller, F.-D.; Plitzko, J. M.; Bramkamp, M.; Thanbichler, M.; Schueler, D.: A gradient-forming MipZ protein mediating the control of cell division in the magnetotactic bacterium Magnetospirillum gryphiswaldense. MOLECULAR MICROBIOLOGY 112 (5), pp. 1423 - 1439 (2019)
Journal Article
Jung, A.; Rassbach, A.; Pulpetta, R. L.; van Teeseling, M. C. F.; Heinrich, K.; Sobetzko, P.; Serrania, J.; Becker, A.; Thanbichler, M.: Two-step chromosome segregation in the stalked budding bacterium Hyphomonas neptunium. NATURE COMMUNICATIONS 10, 3290 (2019)
Journal Article
Billini, M.; Biboy, J.; Kuehn, J.; Vollmer, W.; Thanbichler, M.: A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus. PLOS GENETICS 15 (2), e1007897 (2019)
Journal Article
Kremer, K.; van Teeseling, M. C. F.; Schada v. Borzyskowsi, L.; Bernhardsgrütter, I.; van Spanning, R. J. M.; Gates, A. J.; Remus-Emsermann, M. N. P.; Thanbichler, M.; Erb, T. J.: Dynamic Metabolic Rewiring Enables Efficient Acetyl Coenzyme A Assimilation in Paracoccus denitrificans. MBIO 10 (4), e00805-19 (2019)
Journal Article
Osorio-Valeriano, M.; Altegoer, F.; Steinchen, W.; Urban, S.; Liu, Y.; Bange, G.; Thanbichler, M.: ParB-type DNA Segregation Proteins Are CTP-Dependent Molecular Switches. Cell 179 (7), pp. 1512 - 1524.e15 (2019)
2018
Journal Article
Thanbichler, M.: Cell Division: Symbiotic Bacteria Turn It Upside Down. CURRENT BIOLOGY 28 (7), pp. R306 - R308 (2018)
2017
Journal Article
Lin, L.; Valeriano, M.; Harms, A.; Sogaard-Andersen, L.; Thanbichler, M.: Bactofilin-mediated organization of the ParABS chromosome segregation system in Myxococcus xanthus. Nature Communications 8, 1817 (2017)
Journal Article
Zielinska, A.; Billini, M.; Moll, A.; Kremer, K.; Briegel, A.; Martinez, A.; Jensen, G.; Thanbichler, M.: LytM factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus. Molecular Microbiology 106 (3), pp. 419 - 438 (2017)
Journal Article
Dohlemann, J.; Wagner, M.; Happel, C.; Carrillo, M.; Sobetzko, P.; Erb, T.; Thanbichler, M.; Becker, A.: A Family of Single Copy repABC-Type Shuttle Vectors Stably Maintained in the Alpha-Proteobacterium Sinorhizobium meliloti. ACS Synthetic Biology 6 (6), pp. 968 - 984 (2017)
Journal Article
Cserti, E.; Rosskopf, S.; Chang, Y.; Eisheuer, S.; Selter, L.; Shi, J.; Regh, C.; Koert, U.; Jensen, G.; Thanbichler, M.: Dynamics of the peptidoglycan biosynthetic machinery in the stalked budding bacterium Hyphomonas neptunium. Molecular Microbiology 103 (5), pp. 875 - 895 (2017)
2016
Journal Article
Brenzinger, S.; Dewenter, L.; Delalez, N.J.; Leicht, O.; Berndt, V.; Paulick, A.; Berry, R.M.; Thanbichler, M.; Armitage, J.P.; Maier, B. et al.; Thormann, K.M.: Mutations targeting the plug-domain of the Shewanella oneidensis proton-driven stator allow swimming at increased viscosity and under anaerobic conditions. Molecular Microbiology 102 (5), pp. 925 - 938 (2016)
Journal Article
Muhr, E.; Leicht, O.; Sierra González, S.; Thanbichler, M.; Heider, J.: A Fluorescent Bioreporter for Acetophenone and 1-Phenylethanol derived from a Specifically Induced Catabolic Operon. FRONTIERS IN MICROBIOLOGY 6, 1561 (2016)
2015
Journal Article
Shi, C.; Fricke, P.; Lin, L.; Chevelkov, V.; Wegstroth, M.; Giller, K. M.; Becker, S.; Thanbichler, M.; Lange, A.: Atomic-resolution structure of cytoskeletal bactofilin by solid-state NMR. Sciences Advances 1 (11), e1501087 (2015)
Journal Article
Vasa, S.; Lin, L.; Shi, C.; Habenstein, B.; Riedel, D.; Kuhn, J.; Thanbichler, M.; Lange, A.: beta-Helical architecture of cytoskeletal bactofilin filaments revealed by solid-state NMR. Proceedings of the National Academy of Sciences of the United States of America 112 (2), pp. E127 - E136 (2015)
Journal Article
Jung, A.; Eisheuer, S.; Cserti, E.; Leicht, O.; Strobel, W.; Moll, A.; Schlimpert, S.; Kühn, J.; Thanbichler, M.: Molecular Toolbox for Genetic Manipulation of the Stalked Budding Bacterium Hyphomonas neptunium. Applied and Environmental Microbiology 81 (2), pp. 736 - 744 (2015)
2014
Journal Article
Jia, S.; Keilberg, D.; Hot, E.; Thanbichler, M.; Sogaard-Andersen, L.; Lenz, P.: Effect of the Min System on Timing of Cell Division in Escherichia coli. PLoS ONE 9 (8), e103863 (2014)
Journal Article
Kiekebusch, D.; Thanbichler, M.: Plasmid segregation by a moving ATPase gradient. Proceedings of the National Academy of Sciences of the United States of America 111 (13), pp. 4741 - 4742 (2014)
Journal Article
Strobel, W.; Moll, A.; Kiekebusch, D.; Klein, K.; Thanbichler, M.: Function and Localization Dynamics of Bifunctional Penicillin-Binding Proteins in Caulobacter crescentus. Journal of Bacteriology 196 (8), pp. 1627 - 1639 (2014)
Journal Article
Kiekebusch, D.; Thanbichler, M.: Spatiotemporal organization of microbial cells by protein concentration gradients. Trends in Microbiology 22 (2), pp. 65 - 73 (2014)
2013
Journal Article
Lin, L.; Thanbichler, M.: Nucleotide-independent cytoskeletal scaffolds in bacteria. Cytoskeleton 70 (8), pp. 409 - 423 (2013)
Journal Article
Eun, Y. J.; Zhou, M.; Kiekebusch, D.; Schlimpert, S.; Triyedi, R. R.; Bakshi, S.; Zhong, Z.; Wahlig, T. A.; Thanbichler, M.; Weibel, D. B.: Divin: A Small Molecule Inhibitor of Bacterial Divisome Assembly. Journal of the American Chemical Society 135 (26), pp. 9768 - 9776 (2013)
Journal Article
Klein, E. A.; Schlimpert, S.; Hughes, V.; Brun, Y. V.; Thanbichler, M.; Gitai, Z.: Physiological role of stalk lengthening in Caulobacter crescentus. Communicative & Integrative Biology / Landes BioScience 6 (4), e24561 (2013)
Journal Article
Bulyha, I.; Lindow, S.; Lin, L.; Bolte, K.; Wuichet, K.; Kahnt, J.; van der Does, C.; Thanbichler, M.; Sogaard-Andersen, L.: Two Small GTPases Act in Concert with the Bactofilin Cytoskeleton to Regulate Dynamic Bacterial Cell Polarity. Developmental Cell 25 (2), pp. 119 - 131 (2013)
2012
Journal Article
Schlimpert, S.; Klein, E. A.; Briegel, A.; Hughes, V.; Kahnt, J.; Bolte, K.; Maier, U. G.; Brun, Y. V.; Jensen, G. J.; Gitai, Z. et al.; Thanbichler, M.: General Protein Diffusion Barriers Create Compartments within Bacterial Cells. Cell 151 (6), pp. 1270 - 1282 (2012)
Journal Article
Eun, Y. J.; Foss, M. H.; Kiekebusch, D.; Pauw, D. A.; Westler, W. M.; Thanbichler, M.; Weibel, D. B.: DCAP: A Broad-Spectrum Antibiotic That Targets the Cytoplasmic Membrane of Bacteria. Journal of the American Chemical Society 134 (28), pp. 11322 - 11325 (2012)
Journal Article
Hou, S.; Wieczorek, S. A.; Kaminski, T. S.; Ziebacz, N.; Tabaka, M.; Sorto, N. A.; Foss, M. H.; Shaw, J. T.; Thanbichler, M.; Weibel, D. B. et al.; Nieznanski, K.; Holyst, R.; Garstecki, P.: Characterization of Caulobacter crescentus FtsZ Protein Using Dynamic Light Scattering. Journal of Biological Chemistry 287 (28), pp. 23878 - 23886 (2012)
Journal Article
Kiekebusch, D.; Michie, K. A.; Essen, L.-O.; Loewe, J.; Thanbichler, M.: Localized Dimerization and Nucleoid Binding Drive Gradient Formation by the Bacterial Cell Division Inhibitor MipZ. MOLECULAR CELL 46 (3), pp. 245 - 259 (2012)
Journal Article
Kiekebusch, D.; Michie, K. A.; Essen, L. O.; Lowe, J.; Thanbichler, M.: Localized Dimerization and Nucleoid Binding Drive Gradient Formation by the Bacterial Cell Division Inhibitor MipZ. Molecular Cell 46 (3), pp. 245 - 259 (2012)
2011
Journal Article
Thanbichler, M.: Good things come in small packages: Subcellular organization and development in bacteria. Current Opinion in Microbiology 14 (6), pp. 687 - 690 (2011)
Journal Article
Briegel, A.; Beeby, M.; Thanbichler, M.; Jensen, G. J.: Activated chemoreceptor arrays remain intact and hexagonally packed. Molecular Microbiology 82 (3), pp. 748 - 757 (2011)
2010
Journal Article
Moll, A.; Schlimpert, S.; Briegel, A.; Jensen, G. J.; Thanbichler, M.: DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus. Molecular Microbiology 77 (1), pp. 90 - 107 (2010)
Journal Article
Kühn, J.; Briegel, A.; Mörschel, E.; Kahnt, J.; Leser, K.; Wick, S.; Jensen, G.; Thanbichler, M.: Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus. The EMBO Journal 29, pp. 327 - 339 (2010)
2009
Journal Article
Thanbichler, M.: Synchronization of Chromosome Dynamics and Cell Division in Bacteria. Cold Spring Harbor Perspectives in Biology 2 (1), p. a000331 (2009)
Journal Article
Thanbichler, M.: Spatial regulation in Caulobacter crescentus. Growth and development: prokaryotes, pp. 715 - 721 (2009)
Journal Article
Thanbichler, M.: Closing the Ring: A New Twist to Bacterial Chromosome Condensation. Cell 137, pp. 598 - 600 (2009)
Journal Article
Möll, A.; Thanbichler, M.: FtsN-like proteins are conserved components of the cell division machinery in proteobacteria. Molecular Microbiology 72 (4), pp. 1037 - 1044 (2009)
2008
Journal Article
Radhakrishnan, S. K.; Thanbichler, M.; Viollier, P. H.: The dynamic interplay between a cell fate determinant and a lysozyme homolog drives the asymmetric division cycle of Caulobacter crescentus. Genes & Development 22 (2), pp. 212 - 225 (2008)
Journal Article
Thanbichler, M.; Shapiro, L.: Getting organized--how bacterial cells move proteins and DNA. Nature Reviews Microbiology 6 (1), pp. 28 - 40 (2008)
2007
Journal Article
Thanbichler, M.; Iniesta, A. A.; Shapiro, L.: A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus. Nucleic Acids Research 35, e137, pp. 1 - 16 (2007)
2006
Journal Article
Thanbichler, M.; Shapiro, L.: MipZ, a spatial regulator coordinating chromosome segregation with cell division in Caulobacter. Cell 126, pp. 147 - 162 (2006)
Journal Article
Thanbichler, M.; Shapiro, L.: Chromosome organization and segregation in bacteria. Journal of Structural Biology 156, pp. 292 - 303 (2006)
2005
Journal Article
Thanbichler, M.; Wang, S.; Shapiro, L.: The bacterial nucleoid: A highly organized and dynamic structure. JOURNAL OF CELLULAR BIOCHEMISTRY 96 (3), pp. 506 - 521 (2005)
Journal Article
Gitai, Z.; Thanbichler, M.; Shapiro, L.: The choreographed dynamics of bacterial chromosomes. TRENDS IN MICROBIOLOGY 13 (5), pp. 221 - 228 (2005)
Journal Article
Thanbichler, M.; Viollier, P.; Shapiro, L.: The structure and function of the bacterial chromosome. CURRENT OPINION IN GENETICS & DEVELOPMENT 15 (2), pp. 153 - 162 (2005)
Journal Article
Leibundgut, M.; Frick, C.; Thanbichler, M.; Böck, A.; Ban, N.: Selenocysteine tRNA-specific elongation factor SelB is a structural chimaera of elongation and initiation factors. EMBO JOURNAL 24 (1), pp. 11 - 22 (2005)
2004
Journal Article
Viollier, P.; Thanbichler, M.; McGrath, P.; West, L.; Meewan, M.; McAdams, H.; Shapiro, L.: Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 101 (25), pp. 9257 - 9262 (2004)
Journal Article
Böck, A.; Thanbichler, M.: Selenocysteine. EcoSal Plus 1 (1) (2004)
2003
Journal Article
Thanbichler, M.; Böck, A.: Purification and characterization of hexahistidine-tagged elongation factor SelB. PROTEIN EXPRESSION AND PURIFICATION 31 (2), pp. 265 - 270 (2003)
2002
Journal Article
Thanbichler, M.; Böck, A.: The function of SECIS RNA in translational control of gene expression in Escherichia coli. EMBO JOURNAL 21 (24), pp. 6925 - 6934 (2002)
Journal Article
Thanbichler, M.; Böck, A.: Selenoprotein biosynthesis: purification and assay of components involved in selenocysteine biosynthesis and insertion in Escherichia coli. Methods Enzymol 347, pp. 3 - 16 (2002)
Book Chapter
Böck, A.; Thanbichler, M.; Rother, M.; Resch, A.: Selenocysteine. In: Aminoacyl-tRNA synthetases (Eds. Ibba, M.; Cusack, C.; Francklyn, S.). Landes Bioscienes, Georgetown, USA (2002)
2001
Journal Article
Thanbichler, M.; Böck, A.: Functional analysis of prokaryotic SELB proteins. BIOFACTORS 14 (1-4), pp. 53 - 59 (2001)
2000
Journal Article
Thanbichler, M.; Böck, A.; Goody, R.: Kinetics of the interaction of translation factor SelB from Escherichia coli with guanosine nucleotides and selenocysteine insertion sequence RNA. JOURNAL OF BIOLOGICAL CHEMISTRY 275 (27), pp. 20458 - 20466 (2000)
1999
Journal Article
Neuhierl, B.; Thanbichler, M.; Lottspeich, F.; Böck, A.: A family of S-methylmethionine-dependent thiol/selenol methyltransferases - Role in selenium tolerance and evolutionary relation. JOURNAL OF BIOLOGICAL CHEMISTRY 274 (9), pp. 5407 - 5414 (1999)
Journal Article
Thanbichler, M.; Neuhierl, B.; Böck, A.: S-methylmethionine metabolism in Escherichia coli. JOURNAL OF BACTERIOLOGY 181 (2), pp. 662 - 665 (1999)
1998
Journal Article
Maier, T.; Drapal, N.; Thanbichler, M.; Böck, A.: Strep-tag II affinity purification: An approach to study intermediates of metalloenzyme biosynthesis. ANALYTICAL BIOCHEMISTRY 259 (1), pp. 68 - 73 (1998)
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