Publications

Ermoli, F.; Spahn, C. K.; Sadhir, I.; Bode, H. B.; Diepold, A.: Activation of the Yersinia type III secretion system induces large-scale chromosomal and virulence plasmid DNA rearrangements. bioRxiv: the preprint server for biology, 2025.02.04.636459 (2025)

Lindner, F.; Grossmann, S.; Diepold, A.: Light-controlled secretion and injection of proteins into eukaryotic cells by optogenetic control of the bacterial type III secretion system. In: Optogenetics. Methods in Molecular Biology, Vol. 2840, pp. 115 - 131 (Ed. Baumschlager, A.). Humana, New York, NY (2025)

Lebon, C.; Grossmann, S.; Mann, G.; Lindner, F.; Koide, A.; Koide, S.; Diepold, A.; Hantschel, O.: Cytosolic delivery of monobodies using the bacterial type III secretion system inhibits oncogenic BCR: ABL1 signaling. Cell Communication and Signaling 22 (1), 500 (2024)

Ermoli, F.; Spahn, C. K.; Glatter, T.; Diepold, A.: Yersinia actively downregulates type III secretion and adhesion at higher cell densities. bioRxiv, 2024.06.27.601021 (2024)

Wimmi, S.; Fleck, M.; Helbig, C.; Brianceau, C. F.; Langenfeld, K.; Szymanski, W. G.; Angelidou, G.; Glatter, T.; Diepold, A.: Pilotins are mobile T3SS components involved in assembly and substrate specificity of the bacterial type III secretion system. Molecular Microbiology 121, pp. 304 - 323 (2024)

Diepold, A.: Defining assembly pathways by fluorescence microscopy. In: Bacterial Secretion Systems in Methods in Molecular Biology, Vol. 2715, pp. 383 - 394 (Eds. Journet, L.; Cascales, E.). Humana, New York, NY (2024)

Jakob, S.; Steinchen, W.; Hanßmann, J.; Rosum, J.; Langenfeld, K.; Osorio-Valeriano, M.; Steube, N.; Giammarinaro, P. I.; Hochberg, G. K. A.; Glatter, T. et al.; Bange, G.; Diepold, A.; Thanbichler, M.: The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression. Nature Communications 15 (1), 318 (2024)

Lampaki, D.; Diepold, A.; Glatter, T.: In-depth quantitative proteomics analysis of the Pseudomonas aeruginosa secretome. In: Pseudomonas aeruginosa. Methods in Molecular Biology, Vol. 2721, pp. 197 - 211 (Ed. Bertoni, G.) (2024)

Wimmi, S.; Balinovic, A.; Brianceau, C. F.; Pintor, K. L.; Vielhauer, J.; Turkowyd, B.; Helbig, C.; Fleck, M.; Langenfeld, K.; Kahnt, J. et al.; Glatter, T.; Endesfelder, U.; Diepold, A.: Cytosolic sorting platform complexes shuttle type III secretion system effectors to the injectisome in Yersinia enterocolitica. Nature Microbiology 9, pp. 185 - 199 (2024)

Lampaki, D.; Diepold, A.; Glatter, T.: In-depth quantitative proteomics analysis of the Pseudomonas aeruginosa secretome. In: Pseudomonas aeruginosa: Methods and Protocols in Methods in Molecular Biology, Vol. 2721, pp. 197 - 211 (Eds. Bertoni, G.; Ferrara, S.). Humana New York, NY (2023)

Nikolić, I.; Glatter, T.; Ranković, T.; Berić, T.; Stanković, S.; Diepold, A.: Repertoire and abundance of secreted virulence factors shape the pathogenic capacity of Pseudomonas syringae pv. aptata. Frontiers in Microbiology 14, 1205257 (2023)

Carsten, A.; Rudolph, M.; Weihs, T.; Schmidt, R.; Jansen, I.; Wurm, C. A.; Diepold, A.; Failla, A. V.; Wolters, M.; Aepfelbacher, M.: MINFLUX imaging of a bacterial molecular machine at nanometer resolution. Methods and Applications in Fluorescence 11, 015004 (2023)

Schott, S.; Scheuer, R.; Ermoli, F.; Glatter, T.; Evguenieva-Hackenberg, E.; Diepold, A.: A ParDE toxin–antitoxin system is responsible for the maintenance of the Yersinia virulence plasmid but not for type III secretion-associated growth inhibition. Frontiers in Cellular and Infection Microbiology 13, 166077 (2023)

Prindle, J. R.; Wang, Y.; Rocha, J. M.; Diepold, A.; Gahlmann, A.: Distinct cytosolic complexes containing the type III secretion system ATPase resolved by three-dimensional single-molecule tracking in live Yersinia enterocolitica. Microbiology Spectrum 10 (6), e0174422 (2022)

Unni, R.; Pintor, K. L.; Diepold, A.; Unterweger, D.: Presence and absence of type VI secretion systems in bacteria. Microbiology 168 (4), 001151 (2022)

Gurung, J. M.; Amer, A. A. A.; Chen, S.; Diepold, A.; Francis, M. S.: Type III secretion by Yersinia pseudotuberculosis is reliant upon an authentic N-terminal YscX secretor domain. Molecular Microbiology 117, pp. 886 - 906 (2022)

Lindner, F.; Diepold, A.: Optogenetics in bacteria - applications and opportunities. FEMS Microbiology Reviews 46 (2), fuab055 (2022)

Wimmi, S.; Balinovic, A.; Jeckel, H.; Selinger, L.; Lampaki, D.; Eisemann, E.; Meuskens, I.; Linke, D.; Drescher, K.; Endesfelder, U. et al.; Diepold, A.: Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH. Nature Communications 12 (1), 1625 (2021)

Berger, C.; Ravelli, R. B. G.; Lopez-Iglesias, C.; Kudryashev, M.; Diepold, A.; Peters, P. J.: Structure of the Yersinia injectisome in intracellular host cell phagosomes revealed by cryo FIB electron tomography. Journal of Structural Biology 213 (1), 107701 (2021)

Milne-Davies, B.; Wimmi, S.; Diepold, A.: Adaptivity and dynamics in type III secretion systems. Molecular Microbiology 115 (3), pp. 395 - 411 (2021)

Wimmi, S.; Balinovic, A.; Jeckel, H.; Selinger, L.; Lampaki, D.; Eisemann, E.; Meuskens, I.; Linke, D.; Drescher, K.; Endesfelder, U. et al.; Diepold, A.: Author Correction: Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH. Nature Communications 12 (1), p. 2685 - 2685 (2021)

Lindner, F.; Milne-Davies, B.; Langenfeld, K.; Stiewe, T.; Diepold, A.: LITESEC-T3SS-Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. NATURE COMMUNICATIONS 11 (1), 2381 (2020)

Diepold, A.: Assembly and Post-assembly Turnover and Dynamics in the Type III Secretion System. In: BACTERIAL TYPE III PROTEIN SECRETION SYSTEMS, pp. 35 - 66 (Eds. Wagner, S.; Galan, J. E.). Cham Springer International Publishing (2020)

Lampaki, D.; Diepold, A.; Glatter, T.: A Serial Sample Processing Strategy with Improved Performance for in-Depth Quantitative Analysis of Type III Secretion Events in Pseudomonas aeruginosa. JOURNAL OF PROTEOME RESEARCH 19 (1), pp. 543 - 553 (2020)

Wagner, S.; Diepold, A.: A Unified Nomenclature for Injectisome-Type Type III Secretion Systems. In: BACTERIAL TYPE III PROTEIN SECRETION SYSTEMS, Vol. 427, pp. 1 - 10 (Ed. Wagner, S.). Cham Springer (2020)

Tseytin, I.; Mitrovic, B.; David, N.; Langenfeld, K.; Zarivach, R.; Diepold, A.; Sal-Man, N.: The Role of the Small Export Apparatus Protein, SctS, in the Activity of the Type III Secretion System. FRONTIERS IN MICROBIOLOGY 10, 2551 (2019)

Baker, A. E.; Webster, S. S.; Diepold, A.; Kuchma, S. L.; Bordeleau, E.; Armitage, J. P.; O´Tool, G. A.: Flagellar stators stimulate c-di-GMP production by Pseudomonas aeruginosa. Journal of Bacteriology 201 (18), e00741-18 (2019)

Milne-Davies, B.; Helbig, C.; Wimmi, S.; Cheng, D. W. C.; Paczia, N.; Diepold, A.: Life After Secretion-Yersinia enterocolitica Rapidly Toggles Effector Secretion and Can Resume Cell Division in Response to Changing External Conditions. FRONTIERS IN MICROBIOLOGY 10, 2128 (2019)

Nauth, T.; Huschka, F.; Schweizer, M.; Bosse, J.; Diepold, A.; Failla, A.; Steffen, A.; Stradal, T.; Wolters, M.; Aepfelbacher, M.: Visualization of translocons in Yersinia type III protein secretion machines during host cell infection. PLOS PATHOGENS 14 (12), e1007527 (2018)

Rocha, J.; Richardson, C.; Zhang, M.; Darch, C.; Cai, E.; Diepold, A.; Gahlmann, A.: Single-molecule tracking in live Yersinia enterocolitica reveals distinct cytosolic complexes of injectisome subunits. INTEGRATIVE BIOLOGY 10 (9) (2018)

Rocha, J.; Richardson, C.; Zhang, M.; Diepold, A.; Gahlmann, A.: Cytosolic Assembly Among Bacterial Type 3 Secretion System Proteins Revealed by High-Throughput Single-Molecule Tracking. BIOPHYSICAL JOURNAL 114 (3 Suppl. 1), p. 534A - 534A (2018)

Diepold, A.; Sezgin, E.; Huseyin, M.; Mortimer, T.; Eggeling, C.; Armitage, J.: A dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome. Nature Communications 8, 15940 (2017)

Morgan, J. M.; Duncan, M. C.; Johnson, K. S.; Diepold, A.; Lam, H.; Dupzyk, A. J.; Martin, L. R.; Wong, W. R.; Armitage, J. P.; Linington, R. G. et al.; Auerbuch, V.: Piericidin A1 Blocks Yersinia Ysc Type III Secretion System Needle Assembly. MSPHERE 2 (1), e00030-17 (2017)

Zoued, A.; Diepold, A.: Defining Assembly Pathways by Fluorescence Microscopy. In: Bacterial Protein Secretion Systems. Methods in Molecular Biology, pp. 289 - 298. Humana Press, New York, NY, New York (2017)

Baker, A. E.; Diepold, A.; Kuchma, S. L.; Scott, J. E.; Ha, D. G.; Orazi, G.; Armitage, J. P.; O'Toole, G. A.: PilZ Domain Protein FlgZ Mediates Cyclic Di-GMP-Dependent Swarming Motility Control in Pseudomonas aeruginosa. JOURNAL OF BACTERIOLOGY 198 (13), pp. 1837 - 1846 (2016)

Diepold, A.; Armitage, J. P.: Type III secretion systems: the bacterial flagellum and the injectisome. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 370 (1679), 20150020 (2015)

Diepold, A.; Kudryashev, M.; Delalez, N. J.; Berry, R. M.; Armitage, J. P.: Composition, Formation, and Regulation of the Cytosolic C-ring, a Dynamic Component of the Type III Secretion Injectisome. PLOS BIOLOGY 13 (1), e1002039 (2015)

Gerc, A. J.; Diepold, A.; Trunk, K.; Porter, M.; Rickman, C.; Armitage, J. P.; Stanley-Wall, N. R.; Coulthurst, S. J.: Visualization of the Serratia Type VI Secretion System Reveals Unprovoked Attacks and Dynamic Assembly. CELL REPORTS 12 (12), pp. 2131 - 2142 (2015)

Kudryashev, M.; Diepold, A.; Amstutz, M.; Armitage, J. P.; Stahlberg, H.; Cornelis, G. R.: Yersinia enterocolitica type III secretion injectisomes form regularly spaced clusters, which incorporate new machines upon activation. MOLECULAR MICROBIOLOGY 95 (5), pp. 875 - 884 (2015)

Diepold, A.; Wagner, S.: Assembly of the bacterial type III secretion machinery. FEMS MICROBIOLOGY REVIEWS 38 (4), pp. 802 - 822 (2014)

Kudryashev, M.; Stenta, M.; Schmelz, S.; Amstutz, M.; Wiesand, U.; Castano-Diez, D.; Degiacomi, M. T.; Muennich, S.; Bleck, C. K. E.; Kowal, J. et al.; Diepold, A.; Heinz, D. W.; Dal Peraro, M.; Cornelis, G. R.; Stahlberg, H.: In situ structural analysis of the Yersinia enterocolitica injectisome. ELIFE 2, e00792 (2013)

Diepold, A.; Wiesand, U.; Amstutz, M.; Cornelis, G. R.: Assembly of the Yersinia injectisome: the missing pieces. MOLECULAR MICROBIOLOGY 85 (5), pp. 878 - 892 (2012)

Diepold, A.; Wiesand, U.; Cornelis, G. R.: The assembly of the export apparatus (YscR,S,T,U,V) of the Yersinia type III secretion apparatus occurs independently of other structural components and involves the formation of an YscV oligomer. MOLECULAR MICROBIOLOGY 82 (2), pp. 502 - 514 (2011)

Diepold, A.; Amstutz, M.; Abel, S.; Sorg, I.; Jenal, U.; Cornelis, G. R.: Deciphering the assembly of the Yersinia type III secretion injectisome. EMBO JOURNAL 29 (11), pp. 1928 - 1940 (2010)

Diepold, A.; Li, G.; Lennarz, W. J.; Nuernberger, T.; Brunner, F.: The Arabidopsis AtPNG1 gene encodes a peptide: N-glycanase. PLANT JOURNAL 52 (1), pp. 94 - 104 (2007)