Insights into the small RNA interactome of Sulfolobus acidocaldarius
Graduate Students Mini-Symposium
- Date: Apr 18, 2016
- Time: 01:50 PM (Local Time Germany)
- Speaker: Michael Daume
- MPI / RG Prokaryotic Small RNA Biology
- Location: MPI for Terrestrial Microbiology
- Room: Lecture hall
- Host: Dr. Lennart Randau
- Contact: lennart.randau@mpi-marburg.mpg.de
The
regulation of cellular processes by small RNAs is in many cases mediated by
their interaction with other RNA molecules, e.g. rRNA or mRNA, often
facilitated by RNA-binding proteins. We identified the interaction partners of
the RNA-binding proteins L7Ae and the three LSm proteins by RIP-Seq. The
obtained RNA interactome datasets revealed several examples of RNA-regulated
processes in Archaea. L7Ae was shown to autoregulate its translation by binding
to a Kink-turn motif identified in the 5' UTR of its mRNA. The LSm1 and LSm2
proteins were found to interact with a sRNA involved in biofilm formation.