Streptomyces, in contrast to rod-shaped bacteria, grow by forming hyphae. Their cells extend apically and branch. Moreover, Streptomyces elongated hyphal cells contain several copies of the chromosome. We have addressed the question of how the multiple copies of the chromosome are distributed in Streptomyces cells. To this end, we dissected the role of the segregation proteins (ParA and ParB) and topoisomerase I (TopA). In my talk, I will describe the unique properties of Streptomyces ParA and TopA and explain how they contribute to chromosome arrangement.