Publications

2012

Schick, M., Xie, X., Ataka, K., Kahnt, J., Linne, U. and Shima, S. (2012): Biosynthesis of the iron-guanylylpyridinol cofactor of [Fe]-hydrogenase in methanogenic archaea as elucidated by stable-isotope labeling. J. Am. Chem. Soc. In press.
Shima, S., Krueger, M., Weinert, T. Demmer, U., Kahnt, J., Thauer, R.K. & Ermler, U. (2012): Structure of a methyl-coenzyme M reductase from Black Sea mats that oxidize methane anaerobically. Nature 481, 98-101.
Shima, S., Schick, M., Kahnt, J., Ataka, K., Steinbach, K. & Linne, U. (2012): Evidence for acyl-iron ligation in the active site of [Fe]-hydrogenase provided by mass spectrometry and infrared spectroscopy. Dalton Trans. 41, 767-771.

2011

Kaster, A.-K., Moll, J., Parey, K., & Thauer, R. K. (2011): Coupling of ferredoxin- and heterodisulfide reduction via electron bifurcation in hydrogenotrophic methanogenic archaea. Proc. Natl. Acad. Sci. USA, 108, 2981-2986.
Kaster, A.-K., Goenrich, M., Seedorf,H., Liesegang, H., Wollherr, A., Gottschalk, G., & Thauer, R. K. (2011): More than 200 genes required for methane formation from H₂ and CO₂ and energy conservation in Methanothermobacter marburgensis and M. thermautotrophicus. Archaea, Vol. 2011, Article ID 973848, 1-23 (doi:10.1155/2011/973848)
Gensheimer, M., Brandis-Heep, A., Agarwal S., Thauer, R.K. & Greiner, A. (2011): Polymer/bacteria composite nanofiber nonwoven by electrospinning of living bacteria protected by hydrogel microparticles. Macromolecular Bioscience 11, 333-337.
Thauer R. K. (2011): Anaerobic oxidation of methane with sulfate: on the reversibility of the reactions that are catalyzed by enzymes also involved in methanogenesis from CO₂ (2011) Current opinion in Microbiology 14 (DOI 10.1016/J.mib.2011.03.003)
Buckel W. & Thauer R.K. (2011): Dual Role of S-Adenosylmethionine (SAM+) in the Methylation of sp²-Hybridized Electrophilic carbons. Angewandte Chemie DOI: 10.1002/anie.201105076
Thauer, R. K. (2011): Hydrogenases and the global H₂ cycle (2011) Eur. J. Inorg. Chem. 2011, 919-921.
Thauer, R. K. (2011): A novel mechanism of energetic coupling in anaerobes. Environmental Microbiology Reports 3, 24-25.
Thauer R. K. (2011): Buchbesprechung: Feodor Lynen Sei naiv und mach´ ein Experiment. Biographie des Münchener Biochemikers und Nobelpreisträgers von Heike Will. Angewandte Chemie in press
Thauer R. K. (2011): Schwer zu widerlegen. Dem Mikrobiologen Wolfgang Buckel zum 70. Geburtstag. Marburger UniJournal 36, Seite 56l
Shima, S. & Ermler U. (2011): Structure and function of [Fe]-hydrogenase and its iron- guanylylpyridinol (FeGP) cofactor. Eur. J. Inorg. Chem. 2011, 963-972.
Shima, S., Schick, M. & Tamura, H. (2011): Preparation of [Fe]-hydrogenase from methanogenic archaea. Methods Enzymol. 494, 119-137.
Basen, M., Krüger, M., Milucka, J., Kuever, J., Kahnt, J., Grundmann, O., Meyerdierks, A., Widdel, F. & Shima, S. (2011): Bacterial enzymes for dissimilatory sulfate reduction in a marine microbial mat (Black Sea) mediating anaerobic oxidation of methane. Environ. Microbiol. 13, 1370-1379
Shima, S. & Ataka, K. (2011): Isocyanides inhibit [Fe]-hydrogenase with very high affinity. FEBS Lett. 585, 353-356.

2010

Salomone-Stagni, M., Stellato, F., Whaley, C.M., Vogt, S., Morante, S., Shima, S., Rauchfuss, T.B. & Meyer-Klaucke, W. (2010): The iron structure of [Fe]-hydrogenase and model systems: an X-ray absorption near edge spectroscopy study. Dalton Trans. 39, 3057-3064
Scheller, S., Goenrich, M., Boecher, R., Thauer, R.K. & Jaun, J. (2010): The key nickel enzyme of methanogenesis catalyses anaerobic oxidation of methane Nature 465, 606-609.
Thauer, R. K. (2010): Functionalization of methane in anaerobic microorganisms. Angewandte Chemie, 49, 6712-6713
Scheller, S., Goenrich, M., Mayr, S. Thauer, R. K. & Jaun, B. (2010): Intermediates in the catalytic cycle of methy-coenzyme M reductase: Isotope exchange is consistent with formation of a ?-alkane Ni complex. Angewandte Chemie, 49, 8112-8115.
Shuning W., Haiyan H., Moll, J., &Rudolf K. Thauer (2010): NADP+ reduction with reduced ferredoxin and NADP+ reduction with NADH are coupled via an electron bifurcating enzyme complex in Clostridium kluyveri J. Bacteriol., 192,5115-5123.
Liesegang, H., Kaster, A.-K., Wiezer, A., Goenrich, M., Wollher, A., Seedorf, S., Gottschalk, G. &Thauer, R.K. (2010): Complete Genome Sequence of Methanothermobacter marburgensis, a Methanoarchaeon Model Organism. J. Bacteriol., 192, 5850-5851.
Parey, K., Warkentin, E., Kroneck, P. M. H., & Ermler, U. (2010): Reaction cycle of dissimilatory sulfite reductase from Archaeoglobus fulgidus. Biochemistry 49, 8912-8921.
Shima, S., Vogt, S., Göbels, A. & Bill E. (2010): Iron-chromophore circular dichroism of [Fe]- hydrogenase: the conformational change required for H2 activation. Angew. Chem. Int. Ed. 49, 9917-9921.
Ebner, S., Jaun, B., Goenrich, M., Thauer, R. K. & Harmer, J. (2010): Binding of coenzyme B induces a major conformational change in the active site of methyl-coenzyme M reductase J. Am. Chem. Soc. 132, 567-575
Thauer, R. K., Kaster, A., Goenrich, M., Schick, M., Hiromoto, T. & Shima, S. (2010): Hydrogenases from methanogenic archaea: structure and function, nickel regulation, and H2-storage. Ann. Rev. Biochemistry 79, 507-536.

2009

Jaun, B. and Thauer, R.K (2009): Nickel-alkyl bond formation in the active site of methyl-coenzyme M reductase (MCR). In Metal-carbon bonds in enzymes and cofactors, Vol.6 of Metal Ions in Life Sciences (Sigel, A., Sigel, H., Sigel, R.K.O., eds). John Wiley & Sons, Ltd, Chichester, UK, pp. 115-132
Shima, S., Thauer, R.K. and Ermler, U. (2009): Carbon monoxide as intrinsic ligands to iron in the active site of [Fe]-hydrogenase. In Metal-carbon bonds in enzymes and cofactors, Vol.6 of Metal Ionsin Life Sciences (Sigel, A., Sigel, H., Sigel, R.K.O., eds). John Wiley & Sons, Ltd, Chichester, UK, pp 19-240
Hiromoto, T., Ataka, K., Pilak O., Vogt, S., Stagni, M.S., Meyer-Klaucke, W., Warkentin, E., Thauer, R.K., Shima, S., & Ermler, U. (2009): The crystal structure of C176A mutated [Fe]-hydrogenase suggests an acyl-iron ligation in the active site iron complex. FEBS Letters 583, 585-590
Hiromoto, T., Warkentin, E., Moll, J., Ermler, U., & Shima, S. (2009): The crystal structure of an [Fe]-hydrogenase-Substrate complex reveals the framework for H2 activation. Angew. Chem. Int. Ed. 48, 6457-6460
ter Meulen, V. & Thauer, R. K. (2009): Celebrating Achim Trebst´s 80th birthday. Phoptosynthesis Research 100, 117-119
Ceh, K., Demmer, u.,, Warkentin, E., Thauer, R.K., Shima, S. & Ermler, U. (2009): Structural basis of the hydride transfer mechanism in F420-dependent methylene-tetrahydromethanopterin dehydrogenase. Biochemistry, 48, 10098-10108

2008

Vogt, S., Lyon, E. J., Shima, S. & Thauer, R.K. (2008): The exchange activities of [Fe] hydrogenase (iron-sulfurcluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases. J. Biol. Inorg. Chem. 13, 97-106.
Thauer, R.K. & Shima, S. (2008): Methane as fuel for anaerobic microorganisms. Annals of the New York Academy of Sciences. 1125: 158-170.
Seedorf, H., Fricke, W.F., Veith, B., Brüggemann, H.,Liesegang, H. Strittmatter, A., Miethke, M., Buckel, W., Hinderberger, J., Li, F., Hagemeier,Ch., Thauer, R.K. & Gottschalk, G. (2008): The genome of Clostridium kluyveri, a strict anaerobe with unique metabolic features. Proc. Natl. Acad. Sci. USA. 105 (6):2128-33.
Li, F., Hinderberger, J., Seedorf, H., Zhang, J., Buckel, W. & Thauer, R.K. (2008): Coupled ferredoxin- and crotonyl-CoA reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri. J. Bacteriol. 190, 843-850.
Guo, Y., Wang, H., Xiao, Y., Vogt, S., Thauer, R.K., Shima, S., Volkers, P.I., Rauchfuss, T.B., Pelmenschikov, V., Case, D. A., Alp, E., Sturhahn, W., Yoda, Y. & Cramer, S.P. (2008): Characteriazation of the Fe site in iron-sulfur cluster free hydrogenase (Hmd) and of a model compound via nuclear resonant vibrational spectroscopy (NRVS). Inorganic Chemistry 47, 3969-3977.
Thauer, R. K., Kaster, A.K., Seedorf, H., Buckel, W., and Hedderich, R. (2008): Methanogenic archaea: Ecologically relevant differences in energy conservation. Nature Reviews Microbiology 6, 579-591.
Shima, S., Pilak O., Vogt, S., Schick, M., Stagni, M.S., Meyer-Klaucke, W., Warkentin, E., Thauer, R.K., & Ermler, U. (2008): The crystal structure of [Fe]-hydrogenase reveals the geometry of the active site. Science 321, 572-575..
Harmer, J, Finazzo, C., Piskorski, R., Ebner, S., Duin, E.C., Goenrich, M., Thauer, R.K., Reiher, M., Schweiger, A., Hinderberger, D. and Jaun, B (2008): A nickel hydride complex in the active site of methyl-coenzyme M reductase: Implications for the catalytic cycle. J. Am. Chem. Soc., 130, 10907-10920.
Mayr, S., Latkoczy Ch., Krüger, M., Günther, D., Shima, S., Thauer, R.K., Widdel, F. and Jaun, B. (2008): The structure of a F430 variant from Archaea associated with anaerobic oxidation of methane. J. Am. Chem. Soc., 130, 10758-10767
Hinderberger, D., Ebner, S., Mayr, S., Jaun, B., Reiher, M., Goenrich, M., Thauer, R. K., and Harmer, J. (2008).: Coordination and binding geometry of methyl-coenzyme M in the red1m state of methyl-coenzyme M reductase. J. Biol. Inorg. Chem., 13, 1275-1289.
Thauer, R. K. (2008): Of methanotrophic and Methanogenic Archaea. In: Life Strategies of Microorganisms in the Environment and in Host Organisms (Amann R., Goebel, W., Reinhold-Hurek, B., Schink, B. and Widdel, F. eds). Nova Acta Leopoldina 96, 41-44 (see also pp. 9- 10)
Thauer, R.K. (2008): Biologische Methanbildung: Eine erneuerbare Energiequelle von Bedeutung? In Die Zukunft der Energie (Gruss P. & Schüth F. Herausgeber) Verlag C. H. Beck, pp.119-137.
Thauer, R. K. (2008): Leopoldina als nationale Akademie der Wissenschaften. BIOspektrum 14 (Heft 5), p. 567.
Friedrich, B. and Thauer, R. K. (2008): Molekularfunktion von Katalysatioren bei der Produktion von BioH₂. Jahrbuch 2007 der Deutschen Akademie der Naturforscher Leopoldina (Halle/Saale) LEOPOLDINA 53, 349-350.

2007

Thauer, R. (2007): A fifth pathway of autotrophic CO2-fixation. Science 318,1732-1733.
Seedorf, H., Hagemeier, C.H., Shima, S., Thauer, R.K., Warkentin, E. & Ermler, U. (2007): Structure of coenzyme F420H2 oxidase (FprA), a di-iron flavoprotein from methanogenic Archaea catalyzing the reduction of O2 to H2O. FEBS J. 274, 1588-1599.
Shima, S. & Thauer, R.K. (2007): A third type of hydrogenase catalyzing H2 activaton. The Chemical Record 7 (1), 37-46
Thauer, R.K. & Shima S. (2007): Methyl-coenzyme M reductase in methanogens and methanotrophs. In Archaea, Evolution, Physiology and Molecuar Biology (Garrett, R. & Klenk, H.-P., eds) Blackwell Publishing, Inc. Malden, USA, pp 275-283.
Jaun, B. and Thauer, R.K. (2007): Methyl-coenzmye M reductase and its nickel corphin coenzyme F430 in methanogenic archaea. In Nickel and its Surprising Impact in Nature, Vol. 2 of Metal Ions in Life Sciences (Sigel, A., Sigel, H., Sigel, R.K.O., eds). John Wiley & Sons, Ltd, Chichester, UK, pp 323-356.
Thauer, R.K., Stackebrandt, E. & Hamilton, W.A. (2007): Energy Metabolism of Sulphate Reducing Bacteria. In Sulphate-Reducing Bacteria: Environmental and Engineered Systems (Barton, L.L. & Hamilton, W.A., eds) Cambridge University Press, pp.1-37.
Li, F., Hagemeier, C., Seedorf, H., Gottschalk G. & Thauer, R.K. (2007): Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Sicitrate synthase. J. Bacteriol. 189 (11), 4299-4304.
Kern, D.I., Goenrich, M., Jaun, B., Thauer, R.K., Harmer, J. & Hinderberger, D. (2007): Two sub-states of the red2 state of methyl-coenzyme M reductase revealed by high-field EPR spectrospcopy. J. Biol. Inorg. Chem. 12, 1097-1105.
Kahnt, J., Buchenau, B., Mahlert, F., Krüger, M., Shima, S. & Thauer, R.K. (2007): A thioxopeptide bond and methylated amino acid in the active site region of methyl-coenzyme M reductase from methanogenic archaea. FEBS J. 274, 4913-4921.
Gensheimer, M., Becker, M., Brandis-Heep, A., Wendorff, J.H., Thauer, R.K., Greiner, A. (2007): Novel biohybrid materials by electrospinning: nanofibers of poly(ethylene oxide) and living bacteria. Adv. Mater. 19, 2480-2482.
Forzi, L., Hellwig, P., Thauer, R.K. & Sawers, R.G. (2007): The CO and CN- ligands to the active site Fe in [NiFe]-hydrogenase of Eschericia coli have different metabolic origins. FEBS Lett. 581, 3317-3321.
Forzi, L. & Sawers, G. (2007): Maturation of [NiFe]-hydrogenase in Escherichia coli. Biometals 20, 565-578.