Mechanobiology of membrane remodeling
Dr. Michal Skruzny
Every cell (either microbial, human or eventual artificial one) has to actively reshape its membranes. To understand general principles of membrane reshaping we look at endocytosis in yeast, which provides conserved and well amenable model of membrane remodeling. Specifically, we study how forces are transmitted by endocytic proteins to bend the membrane efficiently. We also characterize the nanoscale architecture of endocytic protein machinery to understand how it evolved towards a robust membrane-remodeling machine. To achieve that, we use advanced fluorescence microscopy (FRET techniques and biosensors, multicolor live-cell imaging), yeast genetics and biochemistry. Our studies should help to formulate biomechanical principles of membrane remodeling not only during endocytosis, but also in other key cellular processes like membrane trafficking, cell morphogenesis and motility, or pathogen invasion.
For more information about our projects and events, please follow us on www.twitter.com/skruznylab
Lizarrondo J, Klebl DP, Niebling S, Abella M, Schroer MA, Mertens HDT, Veith K, Thuenauer R, Svergun DI, Skruzny M, Sobott F, Muench S, Garcia-Alai M (2021).
Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis. Nature Communications 12: 2889 https://doi.org/10.1038/s41467-021-23151-7
Abella M, Andruck L, Malengo G, Skruzny M (2021).
Actin-generated force applied during endocytosis measured by Sla2-based FRET tension sensors. Developmental Cell, 56:2419 10.1016/j.devcel.2021.08.007
Skruzny M*, Pohl E, Gnoth S, Malengo E, Sourjik V (2020).
The protein architecture of the endocytic coat analyzed by FRET microscopy. Molecular Systems Biology 16: e9009. *corresponding author: https://doi.org/10.15252/msb.20199009
Skruzny M, Pohl E, Abella M (2019).
FRET Microscopy in Yeast. Biosensors 9, 122: https://doi.org/10.3390/bios9040122
Garcia-Alai MM*, Heidemann J*, Skruzny M, Gieras A, Mertens HDT, Svergun DI, Kaksonen M, Uetrecht C, Meijers R (2018).
Epsin and Sla2 form assemblies through phospholipid interfaces. Nature Communications 9: 328. *equal contribution: https://doi.org/10.1038/s41467-017-02443-x
Skruzny M*, Desfosses A*, Prinz S, Dodonova SO, Gieras A, Uetrecht C, Jakobi AJ, Abella M, Hagen WJ, Schulz J, Meijers R, Rybin V, Briggs JA, Sachse C, Kaksonen M (2015).
An organized co-assembly of clathrin adaptors is essential for endocytosis. Developmental Cell 33: 150. *equal contribution: https://doi.org/10.1016/j.devcel.2015.02.023
Skruzny M, Brach T, Ciuffa R, Rybina S, Wachsmuth M, Kaksonen, M (2012).
Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Proc Natl Acad Sci USA 109: E2533: https://doi.org/10.1073/pnas.1207011109
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