Intracellular organization & differentiation of bacteria
Dr. Simon Ringgaard
Targeting of cellular components to a particular site in a cell is often a highly regulated process, even in cells as small as bacteria. In bacteria, the proteins regulating as diverse mechanisms as cell division, motility and faithful inheritance of the genome to progeny cells, all require specific and dynamic intracellular localizations that have to be coordinated with the cell cycle. Failure to do so often has detrimental or even lethal consequences. Using the human pathogens Vibrio cholera and Vibrio parahaemolyticus as our model organisms, we study how bacteria control protein localization and establish cellular domains during the cell cycle or in response to environmental changes. In particular, we are interested in understanding how V. parahaemolyticus differentiates from small swimmer cells to large swarmer cells in response to surface contact and how the chemotactic signaling arrays in Vibrio species localize dynamically to the cell poles during the cell cycle. To address these fundamental questions, we use a combination of live cell fluorescence microscopy, genetics, biochemistry and bioinformatics.
Ortega, D.R., Yang, W., Subramanian, P., Mann, P., Kjær, A., Chen, S., Watts, K.J., Pirbadian, S., Collins, D.A., Kooger, R., Kalyuzhnaya, M.G., Ringgaard, S., Briegel, A., Jensen, G.J. (2020)
Repurposing a chemosensory macromolecular machine. Nat Commun. 11:2041. doi: 10.1038/s41467-020-15736-5
Freitas, C., Glatter, T., Ringgaard, S. (2020)
Specific proteomic adaptation to distinct environments in Vibrio parahaemolyticus includes significant fluctuations in expression of essential proteins.Environ Microbiol. (Epub ahead of print). doi:10.1111/1462-2920.14997
Iyer, S.C., Casas-Pastor, D., Kraus, D., Mann, P., Schirner, K., Glatter, T., Fritz, G., Ringgaard, S. (2020)
Transcriptional regulation by σ factor phosphorylation in bacteria. Nat Microbiol. 5:395-406. doi:10.1038/s41564-019-0648-6
Freitas, C., Glatter, T., Ringaard, S. (2020)
The release of a distinct cell type from swarm colonies facilitates dissemination of Vibrio parahaemolyticus in the environment, The ISME Journal.14:230-244. doi: 10.1038/s41396-019-0521-x
Yang W., Alvarado A., Glatter T., Ringgaard S., Briegel A. (2018)
Baseplate variability of Vibrio cholerae chemoreceptor arrays. Proc Natl Acad Sci U S A. 115:13365-13370. doi: 10.1073/pnas.1811931115.
Muraleedharan S, Freitas C, Mann P, Glatter T, Ringgaard S (2018)
A cell length-dependent transition in MinD-dynamics promotes a switch in division-site placement and preservation of proliferating elongated Vibrio parahaemolyticus swarmer cells. Mol Microbiol. 109:365-384. doi: 10.1111/mmi.13996.
Ringgaard S, Yang W, Alvarado A, Schirner K, Briegel A. (2018)
Chemotaxis arrays in Vibrio species and their intracellular positioning by the ParC/ParP system. J Bacteriol. 200:e00793-17. doi: 10.1128/JB.00793-17.
Alvarado, A., Kjær, A., Yang, W., Mann, P., Briegel, A., Waldor, M.K., and Ringgaard, S. (2017)
Coupling chemosensory array formation and localization. Elife. 6:e31058. doi:10.7554/eLife.31058