Dynamic Control of Metabolic Networks
Dr. Hannes Link
Metabolic networks respond rapidly to internal and external perturbations by changing fluxes, metabolite concentrations and enzyme levels. Our group is interested in the regulation of these responses and their functional relevance in microbes. Therefore, we investigate known metabolic control circuits, map novel regulatory interactions and engineer synthetic metabolic regulation. The ultimate goal is to improve metabolically engineered strains by enabling self-optimization of overproduction pathways for chemicals.
Sander T, Farke N, Diehl C, Kuntz M, Glatter T, Link H (2019) Allosteric feedback inhibition enables robust amino acid biosynthesis in E. coli by enforcing enzyme overabundance. Cell Systems 8, 1-10.
Beuter D, Gomes-Filho J, Randau L, Díaz-Pascual F, Drescher K, Link H (2018) Selective enrichment of slow-growing bacteria in a metabolism-wide CRISPRi library with a TIMER protein. ACS Synthetic Biology 7, 2775–2782.
Donati S, Sander T, Link H (2017) Crosstalk between transcription and metabolism: how much enzyme is enough for a cell? WIREs Syst Biol Med 10 (1), e1396.
Guder JC , Schramm T, Sander T, Link H (2017) Time-Optimized Isotope Ratio LC-MS/MS for High-Throughput Quantification of Primary Metabolites. Analytical Chemistry 89 (3): 1624–1631.
Link H, Fuhrer T, Gerosa L, Zamboni N, Sauer U. (2015) Real-time metabolome profiling of the metabolic switch between starvation and growth. Nature Methods 12(11):1091-1097
Link H, Kochanowski K, Sauer U. (2013) Systematic identification of allosteric protein-metabolite interactions that control enzyme activity in vivo. Nature Biotechnology 31(4):357-61.
For a full list of publications see Google Scholar