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DTSTAMP:20240329T055145Z
UID:https://www.mpi-marburg.mpg.de/events/14400/130871
DTSTART:20180628T090000Z
CLASS:PUBLIC
CREATED:20180620T074211Z
DESCRIPTION:Microbes self-organize in microcolonies at solid surfaces while
transitioning to a sessile form within a protective biofilm matrix. Micro
bes also have complex community dynamics at fluid interfaces. While the bi
ological implications of surface-attached and interfacial biofilms for the
environment\, health\, and industry are widely appreciated\, the earlier
developmental stage of microbes as microcolonies has received scant attent
ion. This presentation elucidates two new approaches to investigate microb
ial dynamics in spatially and interfacially confined microsystems. First\,
a new approach to studying microcolony formation and community dynamics i
s described. Using microfluidics-enabled fabrication\, a nanoliter-scale s
essile culture system (the nanoculture) is designed to grow synthet
ic microbial communities. Each nanoculture begins as a several nanoliter d
roplet of suspended cells\, encapsulated by a polydimethylsiloxane (PDMS)
membrane. The physicochemical properties of the encapsulation materials al
low the diffusion of functional probes to interrogate cell physiology unde
r chemical insults\, allowing microbial interactions to be probed within o
r across the confining vessel. We use this versatile platform to investiga
te bacterial-fungal (inter-kingdom) dynamics that play a central role in e
arly childhood dental caries and many infections. Second\, microbial respo
nse to confinement at fluid-fluid interfaces are studied both in terms of
physico-chemical effects and metabolic implications. We study two strains
of P. aeruginosa\, PAO1 and PA14. The PAO1 cells remodel the
hexadecane-water interface to form highly elastic Films of Bacteria at Interfaces (FBI)\, i.e. elastic\, solid films of
bacteria and excreted polysaccharides\, whereas the PA14 cells form activ
e FBI that feature interface-associated microbes that remain highly motile
. Transcriptional profiles of the interfacially confined strains suggest t
hat the elastic FBI provides protection\, in a manner akin to biofilms\, e
nabling cells to cope with the detrimental effects of the interfacial envi
ronment. Together\, these studies provide a basis for new strategies to mi
nimize the deleterious impacts and to optimize the beneficial effects of m
icrobial communities relevant to the environment and health. The nanocultu
re system and FBI-encapsulated droplets can also be exploited in upstream
bioprocessing technologies\, with uses ranging from the encapsulation of b
eneficial microbial communities to high-throughput screening of bioactive
molecules.\nVortragende(r): Prof. Dr. Tagbo Niepa
LAST-MODIFIED:20180626T082328Z
LOCATION:ZSM\, Karl-von-Frisch-Str. 16\, Raum: Seminar Room
ORGANIZER:Prof. Dr. K. Drescher
SUMMARY:Special seminar: Microbial Dynamics at Biointerfaces: Controlling t
he Fate of Microbes under Spatial and Interfacial Confinements
URL:https://www.mpi-marburg.mpg.de/events/14400/130871
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