Graduate Students Mini-Symposium

Establishing CO2 fixation pathways in Methylobacterium extorquens

  • Date: Aug 13, 2018
  • Time: 15:15
  • Speaker: Martina Carrillo
  • MPI Marburg, AG Erb
  • Location: MPI for Terrestrial Microbiology
  • Room: Lecture hall
  • Host: IMPRS
  • Contact: imprs@mpi-marburg.mpg.de

Converting a heterotrophic organism into an autotrophic one is one of the long standing goals of synthetic biology. We focus on Methylobacterium extorquens AM1 as a model organism for implementing autotrophy due to easy separation of energy generation and carbon assimilation. The Calvin‑Benson‑Bassham (CBB) cycle was introduced into M. extorquens leading to a growth phenotype but continuous stable growth was not achieved1. Metabolic 13C-tracer analysis demonstrated the functional operation of the synthetic CBB cycle in this strain. Further engineering has been done to establish laboratory evolution conditions which will yield a stable synthetic autotrophic organism.


1Schada von Borzyskowski, Carrillo et al. An engineered Calvin-Benson-Bassham cycle for carbon dioxide fixation in Methylobacterium extorquens AM1. Metab. Eng. 47, 423–433 (2018).


 
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