engineering often involves the deregulation of overproduction pathways, leading
to higher production rates but at the same time draining resources from native
metabolism. This can result in cells that are less robust against stress.
Synthetic allosteric regulation of enzymes of overproduction pathways could solve
this problem by allowing the cells to regulate the fluxes internally dependent
on the growth conditions. In my project, the goal is to create such enzymes
with synthetic allosteric regulation. As the used methods result in large
libraries of possibly switching enzymes, we furthermore developed a FACS-based
screening method to identify and isolate switching enzymes.