Dr. Ulrike Endesfelder
Ulrike Endesfelder (born 1983)
Diploma in Physics, Bonn University (2008)
PhD (Dr. rer. nat.), Applied Laser Physics and Laser Spectroscopy, Bielefeld University (2012)
Postdoc, Biotechnology and Biophysics, Würzburg University (2012-2013)
Postdoc, Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt (2013-2014)
Project Group Leader at the Department of Systems and Synthetic Microbiology, MPI Marburg (Since 10/2014)
Elected member of the Junge Akademie at the BBAW and Leopoldina 2015
Research Area: Single Molecule Microbiology
The group focuses on the application of techniques from the emerging field of super-resolution microscopy to study microbial cell biology. By combining physical concepts of imaging, photo-manipulation of fluorophores and quantitative read-out analyses, we aim at a single-molecule description of cellular structures and processes.
Single Molecule Localization Microscopy (SMLM) registers the position of individual fluorescent molecules. This provides microscopic images with sub-diffraction spatial resolution approaching 20 nm, and allows resolving tiny cellular structures in space. The single-molecule data can further be used for molecular counting, e.g. of protein copy numbers that may vary with respect to the cell cycle. Localization-based algorithms provide access to sub-populations and heterogeneities that are otherwise hidden through ensemble averaging, e.g. through a spatial analysis of protein distributions or the colocalization of molecules.
We apply recent experimental and analytical strategies to study cellular processes in the model organisms E. coli and S. pombe. We investigate the protein distributions and copy numbers, determine interactions and stoichiometries, and relate these findings with the cell cycle.
Correlative super-resolution imaging of RNA polymerase distribution and dynamics, bacterial membrane and chromosomal structure in E. coli Methods Appl. Fluoresc. 3 (2015) 014005
Ulrike Endesfelder (2014) Advances in Correlative Single-Molecule Localization Microscopy and Electron Microscopy, NanoBioImaging, 1:29-37.
Laura Tarancón Díez, Claudia Bönsch, Sebastian Malkusch, Zinnia Truan, Mihaela Munteanu, Mike Heilemann, Oliver Hartley, Ulrike Endesfelder, Alexandre Fürstenberg (2014) Coordinate-based co-localization mediated analysis of arrestin clustering upon stimulation of the C-C chemokine receptor 5 with RANTES analogues. Histochemistry and Cell Biology, 142(1), 69-77.
Mario Perkovic, Michael Kunz, Ulrike Endesfelder, Stefanie Bunse, Christoph Wigge, Zhou Yu, Victor-Valentin Hodirnau, Margot P. Scheffer, Anja Seybert, Sebastian Malkusch, Erin M. Schuman, Mike Heilemann and Achilleas S. Frangakis (2014). Correlative Light- and Electron Microscopy with chemical tags. Journal of Structural Biology, 186(2), 205-213.
Ulrike Endesfelder and Mike Heilemann (2014). Art and artifacts in single-molecule localization microscopy: beyond attractive images. Nature Methods, 11, 235-238.
Ulrike Endesfelder, Sebastian Malkusch, Franziska Fricke and Mike Heilemann (2014). A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment. Histochemistry and Cell Biology, 141(6), 629-638.
Christoph Spahn, Ulrike Endesfelder and Mike Heilemann (2014). Super-resolution imaging of Escherichia coli nucleoids reveals highly structured and asymmetric segregation during fast growth. Journal of Structural Biology, 185 (3), 243-249.