Dr. Ulrike Endesfelder
Ulrike Endesfelder (born 1983)
Diploma in Physics, Bonn University (2008)
PhD (Dr. rer. nat.), Applied Laser Physics and Laser Spectroscopy, Bielefeld University (2012)
Postdoc, Biotechnology and Biophysics, Würzburg University (2012-2013)
Postdoc, Institute of Physical and Theoretical Chemistry, Goethe-University Frankfurt (2013-2014)
Project Group Leader at the Department of Systems and Synthetic Microbiology, MPI Marburg (Since 10/2014)
Elected member of the Junge Akademie at the BBAW and Leopoldina 2015
Research Area: Single Molecule Microbiology
The group focuses on the application of techniques from the emerging field of super-resolution microscopy to study microbial cell biology. By combining physical concepts of imaging, photo-manipulation of fluorophores and quantitative read-out analyses, we aim at a single-molecule description of cellular structures and processes.
Single Molecule Localization Microscopy (SMLM) registers the position of individual fluorescent molecules. This provides microscopic images with sub-diffraction spatial resolution approaching 20 nm, and allows resolving tiny cellular structures in space. The single-molecule data can further be used for molecular counting, e.g. of protein copy numbers that may vary with respect to the cell cycle. Localization-based algorithms provide access to sub-populations and heterogeneities that are otherwise hidden through ensemble averaging, e.g. through a spatial analysis of protein distributions or the colocalization of molecules.
We apply recent experimental and analytical strategies to study cellular processes in the model organisms E. coli and S. pombe. We investigate the protein distributions and copy numbers, determine interactions and stoichiometries, and relate these findings with the cell cycle.
Bartosz Turkowyd∗, David Virant∗, Ulrike Endesfelder (2016)
From single molecules to life - microscopy at the nanoscale, Analytical and Bioanalytical Chemistry, accepted.
∗Authors contributed equally
Ulrike Endesfelder (2016)
Biospektrum 22(2). doi: 10.1007/s12268-016-0675-2
Matthieu Palayret, Helen Armes, Srinjan Basu, et al. (2015)
Virtual-'Light-Sheet' Single-Molecule Localisation Microscopy Enables Quantitative Optical Sectioning for Super-Resolution Imaging
PLoS ONE 10(4): e0125438. doi: 10.1371/journal.pone.0125438
Christoph Spahn, Francesca Cella-Zannacchi, Ulrike Endesfelder# & Mike Heilemann# (2015)
Correlative super-resolution imaging of RNA polymerase distribution and dynamics, bacterial membrane and chromosomal structure in E. coli
Methods Appl. Fluoresc. 3 014005
Ulrike Endesfelder (2015)
Advances in Correlative Single-Molecule Localization Microscopy and Electron Microscopy